Fast and sensitive. Linear detection range (20 uL sample): 5 to 1000 uM D-sorbitol. Convenient and high-throughput. Room temperature assay. No 37°C incubator required. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Intended Use
OD565nm
Detection Principle
5 uM
Shelf Life
6 months
Specifity
For quantitative determination of D-sorbitol and evaluation of drug effects on sorbitol metabolism.
Reference
Kikuchi, K et al (2020). Diabetic cataract in spontaneously diabetic torii fatty rats. Journal of Diabetes Research, 2020, 3058547. Assay: Sorbitol in rat lens.
Sedlak, L et al (2018). Effect of Resveratrol, a Dietary-Derived Polyphenol, on the Oxidative Stress and Polyol Pathway in the Lens of rats with Streptozotocin-Induced Diabetes. Nutrients, 10(10), 1423. Assay: Sorbitol in rat cell lysate, lens.
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