Fast and sensitive. Linear detection range (20 uL sample): 0.5 to 65 U/L for 20 min reaction at 37°C.Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Intended Use
OD565nm
Detection Principle
0.5 U/L
Shelf Life
6 months
Specifity
For quantitative determination of L-malate dehydrogenase enzyme activity and drug effects on malate dehydrogenase metabolism.
Venkat, S. et al (2017). Genetically encoding thioacetyl-lysine as a non-deacetylatable analog of lysine acetylation in Escherichia coli. FEBS open bio, 7(11), 1805-1814. Assay: Malate dehydrogenase in E.coli cell lysate.
Fan, C et al (2015). rationally evolving tRNAPyl for efficient incorporation of noncanonical amino acids. Nucl. Acids Res. 43(22):e156. Assay: Malate dehydrogenase in E.coli cells.
Trivedi, AK et al (2015). Adaptation of oxidative phosphorylation to photoperiod-induced seasonal metabolic states in migratory songbirds. Comp. Biochem. Physiol. A 184: 34-40. Assay: Malate dehydrogenase in avian plasma.
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