Fast and sensitive. Use of 40 uL sample. Linear detection range 3 to 300 uM lactulose in 96-well plate assay.Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 60 minutes. Room temperature assay. No 37°C heater is needed.High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Intended Use
OD565nm
Detection Principle
3 uM
Shelf Life
6 months
Specifity
For quantitative determination of lactulose in food, beverage, agricultural products, and biological samples
Reference
Szafron, D. (2019). Development of an image based technique for assessment of colorectal epithelial integrity and function in an ovine model (Doctoral dissertation). Assay: Lactulose in horseshoe crab Serum.
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