Fast and sensitive. Linear detection range (20 uL sample): 20 to 5000 uM for 10 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Intended Use
OD565nm
Detection Principle
20 µM
Shelf Life
6 months
Specifity
For quantitative determination of isocitrate and evaluation of drug effects on its metabolism.