Simple and convenient. Both assays require addition of single working reagent and can be completed within 60 minutes. Both assays are performed at room temperature. No 37°C heater is needed.High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Intended Use
OD565nm
Detection Principle
D-Mannitol: 7 uM; Lactulose: 3 uM
Shelf Life
6 months
Specifity
Determination of intestinal permeability through measuring lactulose/mannitol ratio in urine samples.
Reference
Sjostrom, B. et al (2021). Increased intestinal permeability in primary Sjogren's syndrome and multiple sclerosis. Journal of Translational Autoimmunity, 4, 100082. Assay: lactose and mannitol in human urine.
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