Sensitive and accurate. Requires only 20 uL serum sample. Detection limit of 5 mg/dL, linearity up to 300 mg/dL cholesterol in 96-well plate assay.Convenient. Room temperature assay. No 37°C heater is needed.
Intended Use
OD340nm
Detection Principle
5 mg/dL
Shelf Life
6 months
Specifity
For quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism.
Reference
Lu, H et al. (2021). Hepatocyte nuclear factor 4a and glucocorticoid receptor coordinately regulate lipid metabolism in mice fed a high-fat-high-sugar diet. BioRxiv, 2021.02.06.427306. Assay: HDL/LDL in mouse serum.
Poulsen, SS et al. (2021). A transcriptomic overview of lung and liver changes one day after pulmonary exposure to graphene and graphene oxide. Toxicology and Applied Pharmacology, 410, 115343. Assay: HDL/LDL in mouse plasma.
Kim, J et al. (2020). Effects of exercise training and detraining on atheromatous matrix metalloproteinase activity in mice. Atherosclerosis, 299: 15-23. Assay: HDL/LDL in mouse serum.
Mizelman, E et al. (2020). A low-glycemic index, high-fiber, pulse-based diet improves lipid profile, but does not affect performance in soccer players. Nutrients, 12(5), 1324. Assay: HDL/LDL in human serum.
Sudirman, S et al. (2019). A dietary polysaccharide from Eucheuma cottonii downregulates proinflammatory cytokines and ameliorates osteoarthritis-associated cartilage degradation in obese rats. Food & Function, 10(9): 5697-5706. Assay: HDL/LDL in rat serum.
Das, A et al. (2019). Novel interaction of antioxidant-1 with TRAF4: Role in inflammatory responses in endothelial cells. American Journal of Physiology. Cell Physiology, 317(6): C1161-C1171. Assay: HDL/LDL in mouse plasma.
Jager, M., Hubert, A., Gogiraju, R., Bochenek, M. L., Munzel, T., & Schafer, K. (2019). Inducible knockdown of endothelial protein tyrosine phosphatase-1B promotes neointima formation in obese mice by enhancing endothelial senescence. Antioxidants & redox signaling, 30(7), 927-944. Assay: HDL and LDL/VLDL in mice serum.
Sharma, V., Smolin, J., Nayak, J., Ayala, J. E., Scott, D. A., Peterson, S. N., & Freeze, H. H. (2018). Mannose Alters Gut Microbiome, Prevents Diet-Induced Obesity, and Improves Host Metabolism. Cell reports, 24(12), 3087-3098. Assay: HDL and LDL/VLDL in mice serum.
Bergt, S., Grub, A., Wagner, S., Engelke, H., Noldge-Schomburg, G., Vollmar, B. & Wagner, N. M. (2017). Pravastatin but not simvastatin improves survival and neurofunctional outcome after cardiac arrest and cardiopulmonary resuscitation. JACC: Basic to Translational Science, 2(2), 149-159. Assay: HDL and LDL/VLDL in mice plasma.
Shon, S. M., Jang, H. J., Schellingerhout, D., Kim, J. Y., Ryu, W. S., Lee, S. K. & Je, K. H. (2017). Cytokine Response to Diet and Exercise Affects Atheromatous Matrix Metalloproteinase-2/9 Activity in Mice. Circulation Journal 81(10):1528-1536. Assay: HDL and LDL/VLDL in mice serum.
Stanton, Catherine, et al (2017). Exopolysaccharide-producing bacteria, and uses thereof for protecting heart health. U.S. Patent No. 9,808,493 Assay: HDL and LDL/VLDL in mice serum.
Frej, C., Linder, A., Happonen, K. E., Taylor, F. B., Lupu, F., & Dahlback, B. (2016). Sphingosine 1-phosphate and its carrier apolipoprotein M in human sepsis and in Escherichia coli sepsis in baboons. Journal of cellular and molecular medicine, 20(6), 1170-1181. Assay: HDL and LDL/VLDL in baboon plasma.
Ballester-Lozano GF et al. (2015). Comprehensive biometric, biochemical and histopathological assessment of nutrient deficiencies in gilthead sea bream fed semi-purified diets. Br J Nutr. 114(5):713-26. Assay: HDL and LDL/VLDL in fish.
Bourdon JA et al (2012) Hepatic and pulmonary toxicogenomic profiles in mice intratracheally instilled with carbon black nanoparticles reveal pulmonary inflammation, acute phase response, and alterations in lipid homeostasis. Toxicol Sci 127(2):474-84. Assay: HDLandLDL/VLDL in mice plasma.
Shanmugasundaram R & Selvaraj RK (2011). Dietary lutein and fish oil interact to alter atherosclerotic lesions in a Japanese quail model of atherosclerosis. J Anim Physiol Anim Nutr(Berl) 95(6):762-70. Assay: HDL/LDL in mouse blood.
Shon, SM et al (2011). Exercise attenuates matrix metalloproteinase activity in preexisting atherosclerotic plaque. Atherosclerosis 216 (1): 67-73. Assay: HDLandLDL/VLDL in rat serum.
Shon, S-M. et al. (2011). Exercise attenuates matrix metalloproteinase activity in preexisting atherosclerotic plaque. Atherosclerosis 216 (1): 67-73. Assay: HDL and LDL/VLDL in mouse blood.
Uddin MJ et al (2011) Detection of quantitative trait loci affecting serum cholesterol, LDL, HDL, and triglyceride in pigs. BMC Genet 12:62. Assay: HDLandLDL/VLDL in pig serum.
Oliver SR et al (2010). Increased oxidative stress and altered substrate metabolism in obese children. Int J Pediatr Obes. 2010 Oct;5(5):436-44. Assay: HDL/LDL in bird serum.
Ponda MP, et al (2010). Moderate kidney disease inhibits atherosclerosis regression. Atherosclerosis. 210(1):57-62. Assay: HDLandLDL/VLDL in mouse Blood.
Ponda, MP et al (2010). Moderate kidney disease inhibits atherosclerosis regression. Atherosclerosis 210 (1): 57-62. Assay: HDL/LDL in human plasma.
Shanmugasundaram R, Selvaraj RK (2010). Dietary lutein and fish oil interact to alter atherosclerotic lesions in a Japanese quail model of atherosclerosis. J Anim Physiol Anim Nutr (Berl). Epub ahead of print. Assay: HDLandLDL/VLDL in bird serum.
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