Fast and sensitive. Use of 5 µL sample. Linear detection range up to 10 mM L-lactate in 96-well plate assay.Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed.High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Intended Use
OD565nm
Detection Principle
NA
Shelf Life
6 months
Specifity
Direct determination of L-Lactate produced by glycolysis in cell samples. Screening of glycolysis inhibitors.
Reference
Umezu, R., et al. (2020). Macrophage (Drp1) dynamin-related protein 1 accelerates intimal thickening after vascular injury. Arteriosclerosis, Thrombosis, and Vascular Biology, 40(7), e214-e226. Assay: Glycolysis in mouse cell media.
Moschovaki Filippidou, F., et al (2020). Glucagon-like peptide-1 receptor agonism improves nephrotoxic serum nephritis by inhibiting t-cell proliferation. The American Journal of Pathology, 190(2), 400-411 Assay: Glycolysis in mouse T cells.
Bernhart, E., et al (2010). Lysophosphatidic acid receptor activation affects the C13NJ microglia cell line proteome leading to alterations in glycolysis, motility, and cytoskeletal architecture. Proteomics, 10(1), 141-158. Assay: Glycolysis in cell media.
Kusuoka, Osamu, et al (2018). Intermittent calorie restriction enhances epithelial-mesenchymal transition through the alteration of energy metabolism in a mouse tumor model. International journal of oncology 52.2: 413-423. Assay: Glycolysis in mice cells.
Helsel, Aileen R., Melissa J. Oatley, and Jon M. Oatley(2017). Glycolysis-optimized conditions enhance maintenance of regenerative integrity in mouse spermatogonial stem cells during long-term culture. Stem cell reports 8.5: 1430-1441. Assay: Glycolysis in mice cell culture.
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