Sensitive and accurate. Use 20 uL sample. Linear detection range 0.023 - 2 mM glutamine in 96-well plate assay.Convenient. The procedure involves adding a single working reagent, incubation for 40 min at room temperature, adding a stop reagent and reading the optical density. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Intended Use
OD565nm
Detection Principle
23 uM
Shelf Life
6 months
Specifity
For quantitative determination of glutamine and evaluation of drug effects on glutamine metabolism.
Reference
Ishak Gabra, M. B., et al. (2020). Dietary glutamine supplementation suppresses epigenetically-activated oncogenic pathways to inhibit melanoma tumour growth. Nature Communications. 11(1): 3326. Assay: Glutamine in mouse tissue.
Velickovic, K., et al. (2020). Targeting glutamine synthesis inhibits stem cell adipogenesis in vitro. Cellular Physiology and Biochemistry, 54(5), 917-927. Assay: Glutamine in mouse cell extract.
Qu, Y., et al.(2021). Soybean CHX-type ion transport protein GmSALT3 confers leaf Na + exclusion via a root derived mechanism, and Cl - exclusion via a shoot derived process. Plant, Cell & Environment. 44(3): 856-869. Assay: Glutamine in soybean phloem sap.
Stern, R. A., et al. (2019). Ammonia induces a myostatin-mediated atrophy in mammalian myotubes, but induces hypertrophy in avian myotubes. Frontiers in Sustainable Food Systems. 3: 115. Assay: Glutamine in chicken myotube cultures.
Luo, Z., et al. (2020). Co-delivery of 2-Deoxyglucose and a glutamine metabolism inhibitor V9302 via a prodrug micellar formulation for synergistic targeting of metabolism in cancer. Acta Biomaterialia. 105: 239-252. Assay: Glutamine in mouse and human cancer cell extract.
Tran, T. Q., et al. (2020). ?-Ketoglutarate attenuates Wnt signaling and drives differentiation in colorectal cancer. Nature Cancer. 1(3): 345-358. Assay: Glutamine in human colonic tissue.
Dill, Veronika, et al (2019). Investigation of cell culture conditions for optimal foot-and-mouth disease virus production. BMC Biotechnology 19.1: 33. Assay: Glutamine in hamster cells.
Yang, Ying, et al (2019). MiR-135 suppresses glycolysis and promotes pancreatic cancer cell adaptation to metabolic stress by targeting phosphofructokinase-1. Nature communications 10.1: 809. Assay: Glutamine in mice tissues.
Bar-Sagi, Dafna, Cosimo Commisso, and Rengin G. Soydaner-Azeloglu (2018). Cancer diagnostics, therapeutics, and drug discovery associated with macropinocytosis. U.S. Patent No. 9,983,194. Assay: Glutamine in mouse cells.
Basit, Farhan, et al (2018). human dendritic cell subsets undergo distinct metabolic reprogramming for immune response. Frontiers in immunology 9:2489. Assay: Glutamine in human cells.
Ma, Huanrong, et al (2018). Inhibition of SLC1A 5 sensitizes colorectal cancer to cetuximab. International journal of cancer 142.12: 2578-2588. Assay: Glutamine in human cells.
Shen, Guangsi, et al (2018). GOLM1 Stimulation of Glutamine Metabolism Promotes Osteoporosis via Inhibiting Osteogenic Differentiation of BMSCs. Cellular Physiology and Biochemistry 50.5: 1916-1928. Assay: Glutamine in mice cells.
Stern, R. A. (2018). Myogenic Response to Ammonia Differs Between Avian and Mammalian Species. Assay: Glutamine in mouse & chicken cells.
Wang, Yun-Qian, et al (2018). Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner. Nature communications 9.1: 545. Assay: Glutamine in human cells.
Pugh, Jamie N., et al (2017). Glutamine supplementation reduces markers of intestinal permeability during running in the heat in a dose-dependent manner. European journal of applied physiology 117.12: 2569-2577. Assay: Glutamine in human plasma.
Chen, Jianmin, et al (2016). The impact of glutamine supplementation on the symptoms of ataxia-telangiectasia: a preclinical assessment. Molecular neurodegeneration 11.1: 60. Assay: Glutamine in mice blood.
Kempaiah, Prakasha, et al (2016). Reduced Hsp70 and Glutamine in Pediatric Severe Malaria Anemia: Role of hemozoin in Suppressing Hsp70 and NF-kB Activation. Molecular Medicine 22.1: 570-584. Assay: Glutamine in human plasma.
Zhul, M et al (2015). The effects of acute oral glutamine supplementation on exercise-induced gastrointestinal permeability and heat shock protein expression in peripheral blood mononuclear cells. Cell Stress and Chaperones 20(1): 85-93. Assay: Glutamine in human plasma.
Behari, J et al (2014) b-Catenin Links Hepatic Metabolic Zonation with Lipid Metabolism and Diet-Induced Obesity in Mice. The American Journal of Pathology. 184:12. Assay: Glutamine in mice liver cells.
Zhang. M et al (2014), Oral cancer cells may rewire alternative metabolic pathways to survive from siRNA silencing of metabolic enzymes. BMC Cancer 14:223. Assay: Glutamine in human oral keratinocytes.
Zhul, MN et al (2014). Effects of oral glutamine supplementation on exercise-induced gastrointestinal permeability and tight junction protein expression. Journal of Applied Physiology. 116(2):183-191. Assay: Glutamine in human intestinal tissue.
Behjousiar A et al (2012). In situ monitoring of intracellular glucose and glutamine in CHO cell culture. PLoS One 7(4):e34512. Assay: Glutamine in hamster CHO cells.
Qi S et al (2012). Comparison of the metabolic profiling of hepatitis B virus-infected cirrhosis and alcoholic cirrhosis patients by using (1) H NMR-based metabonomics. Hepatol Res 42(7):677-85. Assay: Glutamine in human serum.
Liang, Y et al (2011). Additive effect of tetramethylpyrazine and deferoxamine in the treatment of spinal cord injury caused by aortic cross-clamping in rats. Spinal Cord 49(2):302-6. Assay: Glutamate in rat tissue.
Rink, C et al (2011). Oxygen-inducible glutamate oxaloacetate transaminase as protective switch transforming neurotoxic glutamate to metabolic fuel during acute ischemic stroke. Antioxid Redox Signal 14(10):1777-85. Assay: Glutamate in rat Brain tissue.