Sensitive and accurate. Use as little as 20 uL samples. Linear detection range in 96-well plate for 20 minute incubation at 25°C: 0.02 to 10 U/L glucose oxidase for colorimetric assays and 0.002 to 1.5 U/L for fluorimetric assays.Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature.
Intended Use
OD570nm,FL530/585nm
Detection Principle
0.02 (OD), 0.002U/L (FL).
Shelf Life
6 months
Specifity
For quantitative determination of glucose oxidase activity and evaluation of drug effects on its metabolism.
Reference
Allen, R., Rittmann, B. E., & Curtiss, R. (2019). Axenic Biofilm Formation and Aggregation by Synechocystis sp. Strain PCC 6803 Are Induced by Changes in Nutrient Concentration and Require Cell Surface Structures. Appl. Environ. Microbiol., 85(7), e02192-18. Assay: Glucose oxidase in cyanobacteria cells.
To find more recent publications, please click here.