Sensitive and accurate. Use as little as 20 uL samples. Linear detection range in 96-well plate: 5 to 300 uM (90 ug/dL to 5.4 mg/dL) glucose for colorimetric assays and 1 to 30 uM for fluorimetric assays.Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature.
Intended Use
OD570nm,or FL530/585nm
Detection Principle
5 uM
Shelf Life
6 months
Specifity
For quantitative determination of glucose and evaluation of drug effects on glucose metabolism.
Reference
Lambert, K., et al (2021). Grape polyphenols and exercise training have distinct molecular effects on cardiac hypertrophy in a model of obese insulin-resistant rats. The Journal of Nutritional Biochemistry, 87, 108522. Assay: Glucose in rat blood.
Yohe, T. T., et al (2020). Metabolic and performance responses to the replacement of lactose by fat in milk replacer formulations for dairy calves. Animal: An International Journal of Animal Bioscience, 15(1), 100031. Assay: Glucose in cow serum.
de Carvalho, I. P. C., et al (2021). Increasing preweaning milk replacer supply affects postweaning energy metabolism of Holstein male calves. Animal: An International Journal of Animal Bioscience, 100170. Assay: Glucose in cow serum.
Jeong, S., et al (2021). High fructose drives the serine synthesis pathway in acute myeloid leukemic cells. Cell Metabolism, 33(1), 145-159.e6. Assay: Glucose in mouse cells.
Gulli, J., Kroll, E., & Rosenzweig, F. (2020). Encapsulation enhances protoplast fusant stability. Biotechnology and Bioengineering, 117(6), 1696-1709. Assay: Glucose in yeast media.
Edriss, H., et al (2020). Advanced glycation end products and glycosaminoglycans in patients with diabetic ketoacidosis. Journal of Investigative Medicine: The Official Publication of the American Federation for Clinical Research, 68(3), 738-742. Assay: Glucose in human serum.
Jager, Marianne, et al (2018). Inducible knockdown of endothelial protein tyrosine phosphatase-1B promotes neointima formation in obese mice by enhancing endothelial senescence. Antioxidants & redox signaling 30.7: 927-944. Assay: Glucose in mice serum.
Das, Champak, et al (2017). Multiplexed and fully automated detection of metabolic biomarkers using microdialysis probe. Sensors and Actuators B: Chemical 238: 633-640. Assay: Glucose in glucose.
Klippel, S et al (2012). Methyljasmonate displays in vitro and in vivo activity against multiple myeloma cells. British Journal of Haematology, 159: 340-351. Assay: Glucose in cell line.
Drew BG, et al (2011). Reconstituted high-density lipoprotein infusion modulates fatty acid metabolism in patients with type 2 diabetes mellitus. J Lipid Res. 52(3):572-81. Assay: Glucose in human plasma.
Dhar A, et al (2010). Alagebrium attenuates acute methylglyoxal-induced glucose intolerance in Sprague-Dawley rats. Br J Pharmacol.159(1):166-75. Assay: Glucose in human plasma.
Cha SH et al (2008). Differential effects of central fructose and glucose on hypothalamic malonyl-CoA and food intake. PNAS 105(44):16871-5. Assay: Glucose in mouse blood.
Wolfgang MJ et al (2007). Regulation of hypothalamic malonyl-CoA by central glucose and leptin. PNAS 104(49):19285-90. Assay: Glucose in mouse blood.
Wolfgang MJ et al (2006). The brain-specific carnitine palmitoyltransferase-1c regulates energy homeostasis. PNAS 103(19):7282-7. Assay: Glucose in mouse plasma.
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