Fast and sensitive. Use of 20 µL sample. Linear detection range 0.005 to 2 mM fumarate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density after 30 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Intended Use
OD565nm
Detection Principle
5 µM
Shelf Life
6 months
Specifity
For quantitative determination of fumarate and drug effects on its metabolism.
Reference
Kurabayashi, A et al. (2020). Murine remote ischemic preconditioning suppresses diabetic ketoacidosis by enhancing glycolysis and entry into tricarboxylic acid cycle in the liver. Life Sciences, 253, 117748. Assay: Fumarate in mouse liver.
To find more recent publications, please click here.