Fast and sensitive. Use of 20 uL sample. Linear detection range 0.007 to 3 mM mannitol in 96-well plate assay.Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed.High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Intended Use
OD565nm
Detection Principle
7 uM
Shelf Life
6 months
Specifity
For quantitative determination of D-mannitol in biological, food, beverage and agriculture samples.
Reference
Szafron, D. (2019). Development of an image based technique for assessment of colorectal epithelial integrity and function in an ovine model (Doctoral dissertation). Assay: D-Mannitol in fish Serum.
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