Sensitive. Use 10 uL samples. Linear detection range: colorimetric assay 5 - 1000 uM, fluorimetric assay 3 - 100 uM CoA.Convenient. Room temperature "mix-and-read" procedure can be readily automated for high-throughput assay of thousands of samples per day.
Intended Use
OD570nm,or FL530/585nm
Detection Principle
3 uM
Shelf Life
6 months
Specifity
For quantitative determination of coenzyme A (CoA) and evaluation of drug effects on CoA metabolism.
Reference
Takahashi, Takayuki, Yukitoshi Mine, and Tadashi Okamoto (2018). 2, 3-Dimethoxy-5-methyl-p-benzoquinone (Coenzyme Q0) Disrupts Carbohydrate Metabolism of HeLa Cells by Adduct Formation with Intracellular Free Sulfhydryl-Groups, and Induces ATP Depletion and Necrosis. Biological and Pharmaceutical Bulletin 41.12: 1809-1817. Assay: Coenzyme A in human cells.
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