Fast and sensitive. Linear detection range (20 uL sample): 0.5 to 100 U/L for 20 min reaction.Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handlingsystems for processing thousands of samples per day.
Intended Use
OD565nm
Detection Principle
0.5 U/L
Shelf Life
6 months
Specifity
For quantitative determination of aconitase enzyme activity and high-throughput screening assays for aconitase modulator.
Reference
Stein, C. S., Jadiya, P., Zhang, X., McLendon, J. M., Abouassaly, G. M., Witmer, N. H. & Boudreau, R. L. (2018). Mitoregulin: A lncRNA-encoded microprotein that supports mitochondrial supercomplexes and respiratory efficiency. Cell reports, 23(13), 3710-3720. Assay: Aconitase in human tissue.
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