ELISA Kit for Rat Vascular endothelial growth factor A

Mfr.No.

E0143r

Alternative Names

Vascular permeability factor ; VPF ; Vegf

Categories

Elisa

Function

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis.

Specificity

Natural and recombinant Rat Vascular endothelial growth factor A

Subcellular Location

Secreted VEGF-A120 is acidic and freely secreted. VEGF-A164 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF-A188 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.

Detection Range

31.2-2000pg/mL

Entryname

VEGFA_RAT

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGF-A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	84-93%	79-94%	88-96%	78-91%
EDTA plasma(n=5)	78-89%	82-97%	90-102%	89-103%
heparin plasma(n=5)	83-94%	89-101%	87-99%	91-103%

Method Type

Sandwich

Platform

PF00341

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 4.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 10.2%

Protein Name

Vascular endothelial growth factor A

Recovery

Recovery was determined by spiking various levels of VEGF-A into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	86	80-95
Plasma	95	90-102

Sensitivity

15.84pg/mL

Sequence

50:

MNFLLSWVHW ; TLALLLYLHH ; AKWSQAAPTT ; EGEQKAHEVV ; KFMDVYQRSY ;

100:

CRPIETLVDI ; FQEYPDEIEY ; IFKPSCVPLM ; RCAGCCNDEA ; LECVPTSESN ;

150:

VTMQIMRIKP ; HQSQHIGEMS ; FLQHSRCECR ; PKKDRTKPEK ; KSVRGKGKGQ ;

200:

KRKRKKSRFK ; SWSVHCEPCS ; ERRKHLFVQD ; PQTCKCSCKN ; TDSRCKARQL ;

214:

ELNERTCRCD ; KPRR

Sequence Length

214

String

P16612

Sub Unit

Homodimer; disulfide-linked. Also found as heterodimer with PGF.

Uniprot	P16612
Gene Name	Vegfa
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:83785
Unigene	Rn.1923

Reference	1. "Amino acid and cDNA sequences of a vascular endothelial cell mitogen that is homologous to platelet-derived growth factor." Conn G., Bayne M.L., Soderman D.D., Kwok P.W., Sullivan K.A., Palisi T.M., Hope D.A., Thomas K.A. Proc. Natl. Acad. Sci. U.S.A.87:2628-2633(1990) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM VEGF-A164);PROTEIN SEQUENCE OF 27-190 2. "Developmental expression of vascular endothelial growth factor in the masseter muscle of rats." Ishii H., Oota I., Takuma T., Inomata K. Arch. Oral Biol.46:77-82(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS VEGF-A188; VEGF-A164; VEGF-A144 AND VEGF-A120) 3. "Cloning of multiple VEGF splice variants from hypoxic neonatal rat cardiomyocytes." Marion S., Lee T.-C. Submitted (2001-04) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS VEGF-A164 AND VEGF-A188) strain: Sprague-Dawley. 4. "Purification and characterization of a naturally occurring vascular endothelial growth factor.placenta growth factor heterodimer." DiSalvo J., Bayne M.L., Conn G., Kwok P.W., Trivedi P.G., Soderman D.D., Palisi T.M., Sullivan K.A., Thomas K.A. J. Biol. Chem.270:7717-7723(1995) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 27-40;HETERODIMERIZATION WITH PGF tissue: Glial tumor. 5. "Regulation of VEGF and VEGF receptor expression in the rodent mammary gland during pregnancy, lactation, and involution." Pepper M.S., Baetens D., Mandriota S.J., Di Sanza C., Oikemus S., Lane T.F., Soriano J.V., Montesano R., Iruela-Arispe M.L. Dev. Dyn.218:507-524(2000) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;TISSUE SPECIFICITY;DEVELOPMENTAL STAGE

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ELISA Kit for Rat Vascular endothelial growth factor A

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