ELISA Kit for Rat Urotensin-2B

Mfr.No.

E14913r

Alternative Names

Urotensin II-related peptide ; Urotensin IIB ; U-IIB ; Urotensin-2 domain-containing protein ; Urp ; Uts2d

Categories

Elisa

Function

Potent vasoconstrictor.

Specificity

Natural and recombinant Rat Urotensin-2B

Subcellular Location

Secreted

Detection Range

0.156-10ng/mL

Entryname

UTS2B_RAT

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of UTS2B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	99-108%	102-107%	101-107%	103-106%
EDTA plasma(n=5)	98-106%	97-105%	95-107%	96-107%
heparin plasma(n=5)	95-109%	94-108%	97-105%	99-106%

Method Type

Sandwich

Platform

PF02083

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.6%

Protein Name

Urotensin-2B

Recovery

Recovery was determined by spiking various levels of UTS2B into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	104	101-110
Plasma	102	98-105

Sensitivity

0.067ng/mL

Sequence

50:

MKFFSTSLCF ; GLLALLSVTT ; LLHSVRGRPH ; LSSGHELFPA ; EEHTTQEKLP ;

100:

LGLLIRNPGF ; QRPAHAGVDL ; PSKVEELRQL ; KKLREWFMEA ; KSAEPSNALD ;

118:

KLSSSHPIKR ; ACFWKYCV

Sequence Length

118

Uniprot	Q765I2
Gene Name	Uts2b
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:378939
Unigene	Rn.105611

Reference	1. "Identification of urotensin II-related peptide as the urotensin II-immunoreactive molecule in the rat brain." Sugo T., Murakami Y., Shimomura Y., Harada M., Abe M., Ishibashi Y., Kitada C., Miyajima N., Suzuki N., Mori M., Fujino M. Biochem. Biophys. Res. Commun.310:860-868(2003) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PROTEIN SEQUENCE OF 111-118;FUNCTION;TISSUE SPECIFICITY tissue: Brain. 2. "Characterization of a novel urotensin II precursor: urotensin II B (U2B)." Nothacker H.-P., Civelli O. Submitted (2003-06) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]

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BHE13811914	E14913r	96T	$796		Add to Cart

ELISA Kit for Rat Urotensin-2B

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