ELISA Kit for Rat Tyrosine-protein kinase JAK3

Mfr.No.

E0425r

Alternative Names

Janus kinase 3 ; JAK-3

Categories

Elisa

Function

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.

Specificity

Natural and recombinant Rat Tyrosine-protein kinase JAK3

Subcellular Location

Endomembrane system Peripheral membrane protein Cytoplasm

Detection Range

0.78-50ng/mL

Entryname

JAK3_RAT

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of JAK3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

Method Type

Sandwich

Platform

PF07714

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

Protein Name

Tyrosine-protein kinase JAK3

Recovery

Recovery was determined by spiking various levels of JAK3 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

Sensitivity

0.426ng/mL

Sequence

50:

MAPPSEETPL ; ISQRSCSLSS ; SEAGALHVLL ; PPRGPGPPQR ; LSFSFGDYLA ;

100:

EDLCVRAAKA ; CGILPVYHSL ; FALATEDLSC ; WFPPSHIFSI ; EDVDTQVLVY ;

150:

RLRFYFPGWF ; GLETCHRFGL ; HKDLTSAILD ; VHVLEHLFAQ ; HRSDLVSGRL ;

200:

PVGLSLKDQG ; EFLSLAVLDL ; AQMARKQAQR ; PGELLKSVSY ; KACLPPSLRD ;

250:

LIQGQSFVTR ; RRIRRTVVQA ; LAPCSSLPSR ; PYALMAKYIL ; DLERLHPAAT ;

300:

TESFLVGLPG ; AQEEPGCLRV ; TGDNGIAWSS ; KDQELFQTFC ; DFPEIVDVSI ;

350:

KQAPRVGPAG ; EHRLVTITRM ; DGHILEAEFP ; GLPEALSFVA ; LVDGYFRLIC ;

400:

DSRHFFCKEV ; APPRLLEEEA ; ELCHGPITLD ; FAIHKLKAAG ; SLPGSYILRR ;

450:

SPQDYDSFLL ; TACVQTPLGP ; DYKGCLIRQD ; PSGAFSLVGL ; SQLHRSLQEL ;

500:

LTACWHSGLQ ; VDGTALNLTS ; CCVPRPKEKS ; NLIVVRRGRN ; PTPAPGHSPS ;

550:

CCALTKLSFH ; TIPADSLEWH ; ENLGHGSFTK ; IFHGHRREVV ; DGETHDTEVL ;

600:

LKVMDSRHQN ; CMESFLEAAS ; LMSQVSYPHL ; VLLHGVCMAG ; DSIMVQEFVY ;

650:

LGAIDTYLRK ; RGHLVPASWK ; LQVTKQLAYA ; LNYLEDKGLP ; HGNVSARKVL ;

700:

LAREGVDGNP ; PFIKLSDPGV ; SPTVLSLEML ; TDRIPWVAPE ; CLQEAGTLNL ;

750:

EADKWGFGAT ; TWEVFSGAPM ; HITSLEPAKK ; LKFYEDRGQL ; PALKWTELEG ;

800:

LIAQCMAYDP ; GRRPSFRAIL ; RDLNGLITSD ; YELLSDPTPG ; IPNPRDELCG ;

850:

GAQLYACQDP ; AIFEERHLKY ; ISLLGKGNFG ; SVELCRYDPL ; GDNTGPLVAV ;

900:

KQLQHSGPEQ ; QRDFQREIQI ; LKALHCDFIV ; KYRGVSYGPG ; RQELRLVMEY ;

950:

LPSGCLRDFL ; QRHRARLHND ; RLLLFAWQIC ; KGMEYLGARR ; CVHRDLAARN ;

1000:

ILVESEAHVK ; IADFGLAKLL ; PLGKDYYVVR ; VPGQSPIFWY ; APESLSDNIF ;

1050:

SRQSDVWSFG ; VVLYELFTYS ; DKSCSPSTEF ; LRMIGPEREG ; SPLCHLLELL ;

1100:

Sequence Length

1100

String

Q63272

Sub Unit

Interacts with STAM2 and MYO18A. Interacts with SHB.

Uniprot	Q63272
Gene Name	Jak3
Immunogen Species	Rattus norvegicus (Rat)
Unigene	Rn.11159

Reference	1. "Molecular cloning of rat JAK3, a novel member of the JAK family of protein tyrosine kinases." Takahashi T., Shirasawa T. FEBS Lett.342:124-128(1994) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Spleen.

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ELISA Kit for Rat Tyrosine-protein kinase JAK3

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