ELISA Kit for Rat Thyroglobulin

Mfr.No.

E0355r

Categories

Elisa

Function

Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3).

Specificity

Natural and recombinant Rat Thyroglobulin

Subcellular Location

Secreted

Detection Range

15.6-1000pg/mL

Entryname

THYG_RAT

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
Saliva(n=3)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

Method Type

Sandwich

Platform

PF00135

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

Protein Name

Thyroglobulin

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

Sensitivity

7.8pg/mL

Sequence

50:

MMTLVLWVST ; LLSSVCLVAA ; NIFEYQVDAQ ; PLRPCELQRE ; KAFLKQDEYV ;

100:

PQCSEDGSFQ ; TVQCQNDGQS ; CWCVDSDGTE ; VPGSRQLGRP ; TACLSFCQLH ;

150:

KQRILLSSYI ; NSTDALYLPQ ; CQDSGNYAPV ; QCDLQQVQCW ; CVDTEGMEVY ;

200:

GTRQQGRPTR ; CPRSCEIRSR ; RLLHGVGDKS ; PPQCDADGEF ; MPVQCKFVNT ;

250:

TDMMIFDLIH ; NYNRFPDAFV ; TFSAFRNRFP ; EVSGYCYCAD ; SQGRELAETG ;

300:

LELLLDEIYD ; TIFAGLDQAS ; TFTQSTMYRI ; LQRRFLAIQL ; VISGRFRCPT ;

350:

KCEVEQFTAT ; SFGHPYIPSC ; HRDGHYQTVQ ; CQMERMCWCV ; DAQGIEIPGT ;

400:

RQQGQPLFCA ; KDQSCASERQ ; QALSRLYFET ; PGYFSPQDLL ; SSEDRLVPVS ;

450:

GARLDISCPP ; RIKELFVDSG ; LLRSIAVERY ; QQLSESRSLL ; REAIRAIFPS ;

500:

RELAGLALQF ; TTNPKRLQQN ; LFGGTFLVNA ; AQLNLSGALG ; TRSTFNFSQF ;

550:

FQQFGLPGFL ; VRDRATDLAK ; LLPVSLDSSP ; TPVPLRVPEK ; RVAMNKSVVG ;

600:

TFGFKVNLQE ; NQDALKFLVS ; LMELPEFLVF ; LQRAVSVPED ; RARDLGDVME ;

650:

MVFSAQACKQ ; TSGRFFVPSC ; TAEGSYEDIQ ; CYAGECWCVN ; SQGKEVEGSR ;

700:

VSGGHPRCPT ; KCEKQRAQMQ ; NLAGAQPAGS ; SFFVPTCTSE ; GYFLPVQCFN ;

750:

SECYCVDAEG ; QVIPGTQSTI ; GEPKLCPSVC ; QLQAEQAFLG ; VVGVLLSNSS ;

800:

MVPPISSVYI ; PQCSTSGQWM ; PVQCDGPHEQ ; VFEWYERWNT ; QNSDGQELTT ;

850:

ATLLMKLMSY ; REVASTNFSL ; FLQSLYDAGQ ; QSIFPVLAQY ; PSLQDVPQVV ;

900:

LEGATIQPGE ; NIFLDPYIFW ; QILNGQLSQY ; PGPYSDFSMP ; LEHFNLRSCW ;

950:

CVDEAGQELD ; GTRTRAGEIP ; ACPGPCEEVK ; FRVLKFIKET ; EEIVSASNAS ;

1000:

SFPLGESFLV ; AKGIQLTSEE ; LGLPPLYPSR ; EAFSEKFLRG ; SEYAIRLAAQ ;

1050:

STLTFYQKLR ; ASLGESNGTA ; SLLWSGPYMP ; QCNTIGGWEP ; VQCHPGTGQC ;

1100:

WCVDGWGELI ; PGSLMARSSQ ; MPQCPTSCEL ; SRANGLISAW ; KQAGHQRNPG ;

1150:

PGDLFTPVCL ; QTGEYVRQQT ; SGTGAWCVDP ; SSGEGVPTNT ; NSSAQCPGLC ;

1200:

DALKSRVLSR ; KVGLGYTPVC ; EALDGGFSPV ; QCDLAQGSCW ; CVLASGEEVP ;

1250:

GTRVVGTQPA ; CESPQCPLPF ; SGSDVTDGVV ; FCETASSSGV ; TTVQQCQLFC ;

1300:

RQGLRNVFSP ; GPLICNLESQ ; RWVTLPLPRA ; CQRPQLWQTM ; QTQAHFQLLL ;

1350:

PPGKMCSIDY ; SGLLQAFQVF ; ILDELITRGF ; CQIQVKTFGT ; LVSRTVCDNS ;

1400:

SIQVGCLTAE ; RLGVNATWKL ; QLEDISVGSL ; PNLHSIERAL ; MGQDLLGRFA ;

1450:

NLIQSGKFQL ; HLDSKTFSAD ; TILYFLNGDR ; FVTSPMTQLG ; CLEGFYRVST ;

1500:

TSQDPLGCVK ; CPEGSFSQDG ; KCTPCPAGTY ; QGQAGSSACI ; PCPRGRTTTI ;

1550:

TGAFSKTHCV ; TDCQRDEAGL ; QCDQNGQYQA ; NQKDMDSGEV ; FCVDSEGQRL ;

1600:

QWLQTEAGLS ; ESQCLMMRKF ; EKAPESKVIF ; DASSPVIVKS ; RVPSANSPLV ;

1650:

QCLADCADDE ; ACSFVTVSSM ; SSEVSCDLYS ; WTRDNFACVT ; SDQEEDAVDS ;

1700:

LKETSFGSLR ; CQVKVRNSGK ; DSLAVYVKKG ; HEFTASGQKS ; FEPTGFQNVL ;

1750:

SGLYSSVVFS ; ALGTNLTDTH ; LFCLLACDQD ; SCCDGFIVTQ ; VKEGPTICGL ;

1800:

LSAPDILVCH ; INDWRDASDT ; QANGTCAGVT ; YDQGSRQMTM ; SLGGQEFLQG ;

1850:

LTLLEGTQDS ; FISFQQVYLW ; KDSDIGSRPE ; SMGCGRGMVP ; KSEAPEGADM ;

1900:

ATELFSPVDI ; TQVIVNTSHS ; LPSQQYWLST ; HLFSAEQANL ; WCLSRCAQEP ;

1950:

VFCQLADIME ; SSSLYFTCSL ; YPEAQVCDND ; VESNAKNCSQ ; ILPRQPTALF ;

2000:

QRKVVLNDRV ; KNFYTRLPFQ ; KLSGISIRDR ; IPMSEKLISN ; GFFECERLCD ;

2050:

RDPCCTGFGF ; LNVSQMQGGE ; MTCLTLNSMG ; IQTCSEENGA ; TWRILDCGSE ;

2100:

DTEVHTYPFG ; WYQKPAVWSD ; APSFCPSAAL ; QSLTEEKVAL ; DSWQTLALSS ;

2150:

VIIDPSIKHF ; DVAHISISAT ; RNFSLAQDFC ; LQECSRHQDC ; LVTTLQIQQG ;

2200:

VVRCVFYPDI ; QSCEHSLRSK ; TCWLLLHEEA ; AYIYRKSGAP ; LHQSDGISTP ;

2250:

SVHIDSFGQL ; QGGSQVVKVG ; TAWKQVYQFL ; GVPYAAPPLA ; ENRFQAPEVL ;

2300:

NWTGSWDATK ; LRSSCWQPGT ; RTPTPPQISE ; DCLYLNVFVP ; ENLVSNASVL ;

2350:

VFFHNTVEME ; GSGGQLNIDG ; SILAAVGNLI ; VVTANYRLGV ; FGFLSSGSDE ;

2400:

VAGNWGLLDQ ; VAALTWVQTH ; IGAFGGDPQR ; VTLAADRGGA ; DVASIHLLIT ;

2450:

RPTRLQLFRK ; ALLMGGSALS ; PAAIISPDRA ; QQQAAALAKE ; VGCPNSSVQE ;

2500:

VVSCFRQKPA ; NILNEAQTKL ; LAVSGPFHYW ; GPVVDGQYLR ; ELPSRRLKRP ;

2550:

LPVKVDLLIG ; GSQDDGLINR ; AKAVKQFEES ; QGRTNSKTAF ; YQALQNSLGG ;

2600:

EDSDARILAA ; AIWYYSLEHS ; TDDYASFSRA ; LENATRDYFI ; ICPIVNMASL ;

2650:

WARRTRGNVF ; MYHVPESYGH ; GSLELLADVQ ; YAFGLPFYSA ; YQGYFSTEEQ ;

2700:

SLSLKVMQYF ; SNFIRSGNPN ; YPHEFSQKAA ; EFATPWPDFV ; PGAGGESYKE ;

2750:

LSAQLPNRQG ; LKKADCSFWS ; KYIQTLKDAD ; GAKDAQLTKS ; GEEDLEVGPG ;

2768:

SEEDFSGSLE ; PVPKSYSK

Sequence Length

2768

String

P06882

Sub Unit

Homodimer.

Uniprot	P06882
Gene Name	Tg
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:24826
Unigene	Rn.10429

Reference	1. "A novel missense mutation (G2320R) in thyroglobulin causes hypothyroidism in rdw rats." Hishinuma A., Furudate S., Oh-Ishi M., Nagakubo N., Namatame T., Ieiri T. Endocrinology141:4050-4055(2000) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANT HYPOTHYROIDISM ARG-2320 strain: Wistar Imamichi. 2. "A missense mutation in the thyroglobulin gene causes hypothyroidism and dwarfism not associated with goiter in the WIC-rdw rat." Ding M., Jung C.-C., Cheng J.-M., Miyamoto T., Furudate S.I., Agui T. Submitted (2000-01) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: Fischer 344. 3. "A second thyroglobulin messenger RNA species (rTg-2) in rat thyrocytes." Graves P.N., Davies T.F. Mol. Endocrinol.4:155-161(1990) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-213 strain: Fischer. tissue: Thymocyte. 4. "The complete structure of the rat thyroglobulin gene." Musti A.M., Avvedimento V.E., Polistina C., Ursini V.M., Obici S., Nitsch L., Cocozza S., di Lauro R. Proc. Natl. Acad. Sci. U.S.A.83:323-327(1986) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-59 5. "The sequence of 967 amino acids at the carboxyl-end of rat thyroglobulin. Location and surroundings of two thyroxine-forming sites." di Lauro R., Obici S., Condliffe D., Ursini V.M., Musti A.M., Moscatelli C., Avvedimento V.E. Eur. J. Biochem.148:7-11(1985) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1802-2768

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ELISA Kit for Rat Thyroglobulin

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