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Home / Assay Kits / ELISA Kits

ELISA Kit for Rat SUMO-conjugating enzyme UBC9

SKU : BHE13811307

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E11134r
Alternative Names	RING-type E3 SUMO transferase UBC9 ; SUMO-protein ligase ; Ubiquitin carrier protein 9 ; Ubiquitin carrier protein I ; Ubiquitin-conjugating enzyme E2 I ; Ubiquitin-conjugating enzyme UbcE2A ; Ubiquitin-protein ligase I ; Ubce9
Categories	Elisa
Function	Accepts the ubiquitin-like proteins SUMO1, SUMO2 and SUMO3 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly-SUMO chains. Essential for nuclear architecture and chromosome segregation (By similarity). Necessary for sumoylation of FOXL2 and KAT5.
Specificity	Natural and recombinant Rat SUMO-conjugating enzyme UBC9
Subcellular Location	Nucleus Cytoplasm Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body.
Detection Range	0.312-20ng/mL
Entryname	UBC9_RAT
Method Type	Sandwich
Platform	PF00179
Protein Name	SUMO-conjugating enzyme UBC9
Sensitivity	0.088ng/mL
Sequence	50: MSGIALSRLA ; QERKAWRKDH ; PFGFVAVPTK ; NPDGTMNLMN ; WECAIPGKKG ; 100: TPWEGGLFKL ; RMLFKDDYPS ; SPPKCKFEPP ; LFHPNVYPSG ; TVCLSILEED ; 150: KDWRPAITIK ; QILLGIQELL ; NEPNIQDPAQ ; AEAYTIYCQN ; RVEYEKRVRA ; 158: QAKKFAPS
Sequence Length	158
String	P63281
Sub Unit	Forms a tight complex with RANGAP1 and RANBP2. Interacts with SIAH1, PARP, HIPK1, HIPK2 and PPM1J. Interacts with various transcription factors such as TFAP2A, TFAP2B, TFAP2C, AR, ETS1 and SOX4. Interacts with RWDD3; the interaction enhances the sumoylation of a number of proteins such as HIF1A and I-kappa-B Interacts with FOXL. Forms a complex with SENP6 and UBE2I in response to UV irradiation. Interacts with DNM1l; the interaction Interacts with DNM1l (via its GTPase and B domains); the interaction promotes sumoylation of DNM1L, mainly in its B domain. Interacts with NFATC2IP; this inhibits formation of poly-SUMO chains (By similarity). Interacts with RASD2 AND TCF3.

Uniprot	P63281
Gene Name	Ube2i
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:25573
Unigene	Rn.2274

Reference	1. "Degradation of E2A proteins through a ubiquitin-conjugating enzyme, UbcE2A." Kho C.-J., Huggins G.S., Endege W.O., Hsieh C.-M., Lee M.-E., Haber E. J. Biol. Chem.272:3845-3851(1997) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];INTERACTION WITH TCF3;SUBCELLULAR LOCATION strain: CD Charles River. tissue: Aorta. 2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] tissue: Ovary. 3. "Expression level of Ubc9 protein in rat tissues." Golebiowski F., Szulc A., Sakowicz M., Szutowicz A., Pawelczyk T. Acta Biochim. Pol.50:1065-1073(2003) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TISSUE SPECIFICITY 4. "Rhes, a striatal specific protein, mediates mutant-huntingtin cytotoxicity." Subramaniam S., Sixt K.M., Barrow R., Snyder S.H. Science324:1327-1330(2009) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH RASD2

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SKU	Supplier No.	Size	Your price	Quantity
BHE13811307	E11134r	96T	$796		Add to Cart

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