JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Assay Kits / ELISA Kits

ELISA Kit for Rat Serine/threonine-protein kinase Chk1

SKU : BHE13803127

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

EIAAB SCIENCE INC, WUHAN

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	E11379r
Alternative Names	CHK1 checkpoint homolog ; Checkpoint kinase-1 ; Chk1
Categories	Elisa
Function	Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-508' and phosphorylation of CDC25C creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-283' and 'Ser-296' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51, which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation. Phosphorylates NEK6 and NEK11. Phosphorylation of CDC25A at Ser-76 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11.
Specificity	Natural and recombinant Rat Serine/threonine-protein kinase Chk1
Subcellular Location	Nucleus Cytoplasm Cytoplasm cytoskeleton centrosome Nuclear export is mediated at least in part by XPO1/CRM1. Also localizes to the centrosome specifically during interphase, where it may protect centrosomal CDC2 kinase from inappropriate activation by cytoplasmic CDC25B.
Entryname	CHK1_RAT
Platform	PF00069
Protein Name	Serine/threonine-protein kinase Chk1
Sequence	50: MAVPFVEDWD ; LVQTLGEGAY ; GEVQLAVNRI ; TEEAVAVKIV ; DMKRAIDCPE ; 100: NIKKEICINK ; MLNHENVVKF ; YGHRREGNIQ ; YLFLEYCSGG ; ELFDRIEPDI ; 150: GMPEQDAQRF ; FHQLMAGVVY ; LHGIGITHRD ; IKPENLLLDE ; RDNLKISDFG ; 200: LATVFRHNNR ; ERLLNKMCGT ; LPYVAPELLK ; RKEFHAEPVD ; VWSCGIVLTA ; 250: MLAGELPWDQ ; PSDSCQEYSD ; WKEKKTYLNP ; WKKIDSAPLA ; LLHKILVENP ; 300: SARITIPDIK ; KDRWYNKPLN ; RGAKRPRATS ; GGMSESSSGF ; SKHIHSNLDF ; 350: SPINSGSSEE ; NVKFSSSQPE ; PRTGLSLWDT ; GPSNVDKLVQ ; GISFSQPTCP ; 400: DHMLVNSQLL ; GTPGSSQNPW ; QRLVKRMTRF ; FTKLDADKSY ; QCLKETFEKL ; 450: GYQWKKSCMN ; QVTVSTMDRR ; NNKLIFKINL ; VEMDEKILVD ; FRLSKGDGLE ; 476: FKRHFLKIKG ; KLSDIVSSQK ; VWFPVT
Sequence Length	476
String	Q91ZN7
Sub Unit	Interacts with BRCA1, CLSPN, FBXO6, PPM1D, RAD51, TIMELESS, XPO1/CRM1 and YWHAZ/14-3-3 zeta (By similarity). Interacts with FEM1B.

Uniprot	Q91ZN7
Gene Name	Chek1
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:140583
Unigene	Rn.33267

Reference	1. "Cloning and characterization of liver-specific isoform of Chk1 gene from rat." Shann Y.-J., Hsu M.-T. J. Biol. Chem.276:48863-48870(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2);ALTERNATIVE PROMOTER USAGE;TISSUE SPECIFICITY tissue: Liver. 2. "The rat homolog of checkpoint kinase chk1." Wang X., Wang Y. Submitted (2001-11) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1) strain: Fischer 344.

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHE13803127	E11379r	96T	$796		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.