PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Specificity
Natural and recombinant Rat Protein kinase C epsilon type
Entryname
KPCE_RAT
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KPCE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤5.4%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤8.3%
Protein Name
Protein kinase C epsilon type
Recovery
Recovery was determined by spiking various levels of KPCE into serum and plasma .
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Reviews of ELISA Kit for Rat Protein kinase C epsilon type