ELISA Kit for Rat Polymeric immunoglobulin receptor

Mfr.No.

E1286r

Categories

Elisa

Function

This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.

Specificity

Natural and recombinant Rat Polymeric immunoglobulin receptor

Subcellular Location

Secretory component Secreted

Detection Range

0.156-10ng/mL

Entryname

PIGR_RAT

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PIgR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	85-98%	80-96%	93-101%	93-105%
EDTA plasma(n=5)	90-101%	85-94%	89-96%	87-99%
heparin plasma(n=5)	83-94%	90-102%	94-103%	92-101%

Method Type

Sandwich

Platform

PF07686

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.5

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.1

Protein Name

Polymeric immunoglobulin receptor

Recovery

Recovery was determined by spiking various levels of PIgR into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	88	79-99
Plasma	95	90-103

Sensitivity

0.078ng/mL

Sequence

50:

MRLSLFALLV ; TVFSGVSTQS ; PIFGPQDVSS ; IEGNSVSITC ; YYPDTSVNRH ;

100:

TRKYWCRQGA ; NGYCATLISS ; NGYLSKEYSG ; RASLINFPEN ; STFVINIAHL ;

150:

TQEDTGSYKC ; GLGTTNRGLF ; FDVSLEVSQV ; PEFPNDTHVY ; TKDIGRTVTI ;

200:

ECRFKEGNAH ; SKKSLCKKRG ; EACEVVIDST ; EYVDPSYKDR ; AILFMKGTSR ;

250:

DIFYVNISHL ; IPSDAGLYVC ; QAGEGPSADK ; NNADLQVLEP ; EPELLYKDLR ;

300:

SSVTFECDLG ; REVANDAKYL ; CRKNKETCDV ; IINTLGKRDP ; AFEGRILLTP ;

350:

RDDNGRFSVL ; ITGLRKEDAG ; HYQCGAHSSG ; LPQEGWPVQA ; WQLFVNEEST ;

400:

IPNSRSVVKG ; VTGGSVAIVC ; PYNPKESSSL ; KYWCHWEADE ; NGRCPVLVGT ;

450:

QALVQEGYEG ; RLALFDQPGS ; GAYTVILNQL ; TTQDSGFYWC ; LTDGDSRWRT ;

500:

TIELQVAEAT ; KKPDLEVTPQ ; NATAVIGETF ; TISCHYPCKF ; YSQEKYWCKW ;

550:

SNDGCHILPS ; HDEGARQSSV ; SCDQSSQIVS ; MTLNPVKKED ; EGWYWCGVKE ;

600:

GQVYGETTAI ; YVAVEERTRG ; SPHINPTDAN ; ARAKDAPEEE ; AMESSVREDE ;

650:

NKANLDPRLF ; ADEREIQNAG ; DQAQENRASG ; NAGSAGGQSG ; SSKVLFSTLV ;

700:

PLGLVLAVGA ; VAVWVARVRH ; RKNVDRMSIS ; SYRTDISMGD ; FRNSRDLGGN ;

750:

DNMGATPDTQ ; ETVLEGKDEI ; ETTTECTTEP ; EESKKAKRSS ; KEEADMAYSA ;

769:

FLFQSSTIAA ; QVHDGPQEA

Sequence Length

769

String

P15083

Uniprot	P15083
Gene Name	Pigr
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:25046
Unigene	Rn.234069

Reference	1. "Intracellular targeting signals of polymeric immunoglobulin receptors are highly conserved between species." Banting G., Brake B., Braghetta P., Luzio J.P., Stanley K.K. FEBS Lett.254:177-183(1989) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Liver.

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BHE13808629	E1286r	96T	$796		Add to Cart

ELISA Kit for Rat Polymeric immunoglobulin receptor

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