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Home / Assay Kits / ELISA Kits

ELISA Kit for Rat Podocalyxin

SKU : BHE13809363

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E0768r
Alternative Names	Podocalyxin-like protein 1 ; PC ; Pclp1
Categories	Elisa
Function	Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.
Specificity	Natural and recombinant Rat Podocalyxin
Subcellular Location	Apical cell membrane Cell projection microvillus Membrane raft Cell projection lamellipodium Cell projection filopodium Cell projection ruffle Membrane Single-pass type I membrane protein In single attached epithelial cells is restricted to a preapical pole on the free plasma membrane whereas other apical and basolateral proteins are not yet polarized. Colocalizes with SLC9A3R2 at the apical plasma membrane during epithelial polarization. Colocalizes with SLC9A3R1 at the trans-Golgi network (transiently) and at the apical plasma membrane. Its association with the membrane raft is transient. Forms granular, punctuated pattern, forming patches, preferentially adopting a polar distribution, located on the migrating poles of the cell or forming clusters along the terminal ends of filipodia establishing contact with the endothelial cells. Colocalizes with the submembrane actin of lamellipodia, particularly associated with ruffles. Colocalizes with vinculin at protrusions of cells. Colocalizes with ITGB1. Colocalizes with actin filaments, EZR and SLC9A3R1 in a punctate pattern at the apical cell surface where microvilli form (By similarity). Colocalizes with EZR and SLC9A3R2 at the apical cell membrane of glomerular epithelium cells. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, RAB11A and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis.
Detection Range	78-5000 pg/mL
Entryname	PODXL_RAT
Method Type	Sandwich
Platform	PF06365
Protein Name	Podocalyxin
Sensitivity	36 pg/mL
Sequence	50: MRPTLALSAL ; LLLQLLLLST ; PSLSQDNGNK ; TDTSDITSID ; QNQDKPATNQ ; 100: PSNATPKSSV ; QPPTPTSIST ; SSPDPKATQS ; SNSSVTTTSD ; STTDRTSSST ; 150: STVPTTSNSG ; QTVSSGGKSS ; DKITTALPTT ; LGPVNASSQP ; TDLNTSTKLP ; 200: STPTTNSTAS ; PHQPVSHSEG ; QHTTVQSSSA ; SVSSSDNTTL ; LWILTTSKPT ; 250: GTSEGTQPIA ; ISTPGITTPV ; STPLQPTGSP ; GGTESVPTTE ; EFTHSTSSWT ; 300: PVVSQGPSTP ; SSTWTSGSYK ; LKCDPAIKPH ; EELLILNLTR ; DSFCKGSPPN ; 350: ERFLELLCHS ; AKASFKPAED ; SCALELAPIL ; DNQAVAVKRI ; VIETKLSPKA ; 400: VFELLKDKWD ; DLTEAGVIDI ; HLGKEGPPEV ; NEDRFSLPLI ; ITIVCMASFL ; 450: LLVAALYGCC ; HQRISQRKDQ ; QRLTEELQTV ; ENGYHDNPTL ; EVMETPSEMQ ; 485: EKKVVNLNGE ; LGDSWIVPLD ; NLTKEDLDEE ; EDTHL
Sequence Length	485
String	Q9WTQ2
Sub Unit	Monomer; when associated with the membrane raft. Oligomer; when integrated in the apical membrane. Interacts with SLC9A3R2. Interacts (via the C-terminal PDZ-binding motif DTHL) with SLC9A3R1 (via the PDZ domains); the interaction take place early in the secretory pathway and is necessary for its apical membrane sorting (By similarity). Found in a complex with EZR, PODXL and SLC9A3R2. Associates with the actin cytoskeleton through complex formation with EZR and SLC9A3R2. Interacts (via the C-terminal PDZ-binding motif DTHL) with SLC9A3R1 (via the PDZ domains); interaction is not detected in glomerular epithelium cells. Interacts (via the C-terminal PDZ-binding motif DTHL) with SLC9A3R2 (via the PDZ 1 domain); interaction is detected in glomerular epithelium cells. Interacts with EZR.

Uniprot	Q9WTQ2
Gene Name	Podxl
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:192181
Unigene	Rn.162741

Reference	1. "Rat podocalyxin." Kobayashi T. Submitted (1998-12) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] 2. "Expression of podocalyxin inhibits cell-cell adhesion and modifies junctional properties in Madin-Darby canine kidney cells." Takeda T., Go W.Y., Orlando R.A., Farquhar M.G. Mol. Biol. Cell11:3219-3232(2000) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];FUNCTION 3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] tissue: Lung. 4. "Loss of glomerular foot processes is associated with uncoupling of podocalyxin from the actin cytoskeleton." Takeda T., McQuistan T., Orlando R.A., Farquhar M.G. J. Clin. Invest.108:289-301(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH EZR; SLC9A3R1 AND SLC9A3R2;SUBCELLULAR LOCATION;TISSUE SPECIFICITY

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SKU	Supplier No.	Size	Your price	Quantity
BHE13809363	E0768r	96T	$796		Add to Cart

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