ELISA Kit for Rat Meteorin

Mfr.No.

E13481r

Alternative Names

Hypoxia/reoxygenation regulatory factor ; Hyrac

Categories

Elisa

Function

Involved in both glial cell differentiation and axonal network formation during neurogenesis. Promotes astrocyte differentiation and transforms cerebellar astrocytes into radial glia. Also induces axonal extension in small and intermediate neurons of sensory ganglia by activating nearby satellite glia.

Specificity

Natural and recombinant Rat Meteorin

Subcellular Location

Secreted

Detection Range

78-5000pg/mL

Entryname

METRN_RAT

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

Protein Name

Meteorin

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

Sensitivity

39.3pg/mL

Sequence

50:

MLVAALLCAL ; CCGLLAASAR ; AGYSEDRCSW ; RGSGLTQEPG ; SVGQLTLDCT ;

100:

EGAIEWLYPA ; GALRLTLGGS ; DPGTRPSIVC ; LRPTRPFAGA ; QVFAERMAGN ;

150:

LELLLAEGQG ; LAGGRCMRWG ; PRERRALFLQ ; ATPHRDISRR ; VAAFQFELHE ;

200:

DQRAEMSPQA ; QGFGVDGACR ; PCSDAELLLT ; ACTSDFVIHG ; TIHGVVHDME ;

250:

LQESVITVVA ; TRVIRQTLPL ; FQEGSSEGRG ; QASVRTLLRC ; GVRPGPGSFL ;

291:

FMGWSRFGEA ; WLGCAPRFQE ; FSRVYSAALA ; AHLNPCEVAL ; D

Sequence Length

291

Uniprot	Q5Q0T9
Gene Name	Metrn
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:287151
Unigene	Rn.50391

Reference	1. Park J.A., Song H.S., Kim K.-W. Submitted (2004-10) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: Sprague-Dawley. tissue: Brain.

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BHE13806332	E13481r	96T	$796		Add to Cart

ELISA Kit for Rat Meteorin

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