ELISA Kit for Rat Leucine-rich PPR motif-containing protein, mitochondrial

Mfr.No.

E14393r

Alternative Names

130 kDa leucine-rich protein ; LRP 130 ; Leucine rich protein 157 ; rLRP157 ; Lrp157

Categories

Elisa

Function

May play a role in RNA metabolism in both nuclei and mitochondria. In the nucleus binds to HNRPA1-associated poly(A) mRNAs and is part of nmRNP complexes at late stages of mRNA maturation which are possibly associated with nuclear mRNA export. May bind mature mRNA in the nucleus outer membrane. In mitochondria binds to poly(A) mRNA. Plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. May be involved in transcription regulation. Cooperates with PPARGC1A to regulate certain mitochondrially encoded genes and gluconeogenic genes and may regulate docking of PPARGC1A to transcription factors. Seems to be involved in the transcription regulation of the multidrug-related genes MDR1 and MVP. Part of a nuclear factor that binds to the invMED1 element of MDR1 and MVP gene promoters. Binds single-stranded DNA.

Specificity

Natural and recombinant Rat Leucine-rich PPR motif-containing protein, mitochondrial

Subcellular Location

Mitochondrion Nucleus Nucleus nucleoplasm Nucleus inner membrane Nucleus outer membrane

Detection Range

0.156-10ng/mL

Entryname

LPPRC_RAT

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	88-103%	88-101%	91-105%	89-103%
EDTA plasma(n=5)	87-97%	91-103%	92-101%	88-105%
heparin plasma(n=5)	90-104%	94-108%	101-106%	91-109%

Method Type

Sandwich

Platform

PF01535

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 5.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 8.7%

Protein Name

Leucine-rich PPR motif-containing protein, mitochondrial

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	93-102
Plasma	99	95-105

Sensitivity

0.078ng/mL

Sequence

50:

MSALLRPARW ; LLGAAAVPRL ; PLSLRLPAGG ; PGRLPSVVRV ; AAAGGRPAAG ;

100:

ELLSQARLYA ; IVAEKKDLPE ; EPAPVRRSGS ; QFDWALMRLD ; NSVRRTGRIT ;

150:

KGLLQKVFES ; TCRSGSPGSN ; QALLLLRSCG ; SLLPELSLAE ; RTEFAHKIWD ;

200:

KLQQLGTVYD ; VSHYNALLKV ; YLQNEYRFSP ; TDFLAKMEGA ; NIQPNRVTYQ ;

250:

RLIAAYCSVG ; DIEGASKILG ; FMKTRDLPIT ; EAVFSALVTG ; HARAGDMESA ;

300:

ENILTVMKQA ; GIEPGPDTYL ; ALLNAHAEKG ; DIDHVKQILE ; KVEKSDHYFM ;

350:

DRDFLQIIVS ; FSKAGYPQYV ; SEILEKITYE ; RRSIPDAMNL ; ILLLVTEKLE ;

400:

DTAFQVLLAL ; PLARDETSSS ; FGSFFLRHCV ; TMDTPAEKLI ; DYCKRLRDAK ;

450:

VHSSSLQFTL ; HCALQANKTA ; LAKAVMEALR ; DEGFPIRTHY ; FWPLLVGHQK ;

500:

TKNVQGIIDI ; LKIMKEMGVD ; PDQETYINYV ; FPCFGSVQSA ; RAALQENKCL ;

550:

PKSTTFAQAE ; VRNEAINGNL ; QNILSFLESN ; ALPFSFNSLR ; GSLILGFRRS ;

600:

MNIDLWSKIT ; ELLYKDDRYC ; QKPPGPTEAV ; GYFLYNLIDS ; MSDSEVQAKE ;

650:

ERLRQYFHQL ; REMNVKVSEN ; IYKGICNLLD ; NYHVPELIKD ; VKVLVDREKI ;

700:

DSRKTSQFTS ; SDLESTLEKL ; KAEGHPVGDP ; LKQLILLLCS ; EENMQKALEV ;

750:

KAKYESDMVI ; GGYAALINLC ; CRHDNAEDAL ; NLKQEFDRLD ; PSAVLDTAKY ;

800:

VALVKVLGKH ; GRVQDAINIL ; KEMKEKDVVI ; KDAAVLSFFH ; ILNGAALRGE ;

850:

IETVKQLHEA ; IVTLGLAKPS ; SNISFPLVTV ; HLEKDDLPAA ; LEASIACHEK ;

900:

YKVLPRIHDV ; LCKLIEKGET ; DLIQKAMDFV ; SQEQGEMSML ; YDLFFAFLQT ;

950:

GNYKEAKKII ; ETPGIRARPT ; RLQWFCDRCI ; ANNQVETLEK ; LVELTEKLFE ;

1000:

CDRDQMYYNL ; LKLYKISGDW ; QRADAVWNKM ; QEENLIPRER ; TLRLLAGILK ;

1050:

TSNQEVPFDV ; PELWFGDDRS ; SLSSSSPSAG ; DTVTEKMLLS ; DCRLKKSKDA ;

1100:

YNIFLKAEKQ ; DVVFSSEAYS ; TLVGLLLSKD ; DFTRAMHVKD ; FAETHIKGFT ;

1150:

LNGAASSLLI ; IAQVRRDYLK ; VALETLKAAL ; DLEQVPSELA ; VTRLIQALAL ;

1200:

QGDVKSIETI ; QKMVKGLDAI ; ELSRMVFINN ; IALAQMKNNE ; IDAAIENIEH ;

1250:

MLASENQTVE ; HQYFGLSYLF ; RKVIEEQMEP ; ALEKLSIMSE ; RLANQFALYK ;

1300:

PVTDLFLQLV ; DSGKVDEARA ; LLERCGAIAE ; QTSILSVFCL ; RTSQKPKKAP ;

1350:

VLKTLLELIP ; ELRENDRVYS ; CSMKSYVADK ; DVASAKALYE ; HLTAKNMKLD ;

1392:

DLFLKRYASL ; LKDVGEPVPF ; TEPPESFGFY ; IKQLKEAREN ; PS

Sequence Length

1392

String

Q5SGE0

Sub Unit

Interacts with CECR2, HEBP2, MAP1S, UXT, PPARGC1A and FOXO1 (By similarity). Component of mRNP complexes associated with HNRPA1.

Uniprot	Q5SGE0
Gene Name	Lrpprc
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:313867
Unigene	Rn.92051

Reference	1. "Isolation of an mRNA binding protein homologue that is expressed in nociceptors." Eilers H., Trilk S.L., Lee S.Y., Xue Q., Jong B.E., Moff I., Levine J.D., Schumacher M.A. Eur. J. Neurosci.20:2283-2293(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];SUBCELLULAR LOCATION;TISSUE SPECIFICITY;INDUCTION

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ELISA Kit for Rat Leucine-rich PPR motif-containing protein, mitochondrial

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