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Home / Assay Kits / ELISA Kits

ELISA Kit for Rat Insulin receptor substrate 1

SKU : BHE13805595

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E1660r
Alternative Names	pp185 ; Irs-1
Categories	Elisa
Function	May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
Specificity	Natural and recombinant Rat Insulin receptor substrate 1
Detection Range	0.312-20ng/mL
Entryname	IRS1_RAT
Method Type	Sandwich
Platform	PF02174
Protein Name	Insulin receptor substrate 1
Sensitivity	0.174ng/mL
Sequence	50: MASPPDTDGF ; SDVRKVGYLR ; KPKSMHKRFF ; VLRAASEAGG ; PARLEYYENE ; 100: KKWRHKSSAP ; KRSIPLESCF ; NINKRADSKN ; KHLVALYTRD ; EHFAIAADSE ; 150: AEQDSWYQAL ; LQLHNRAKAH ; HDGAGGGCGG ; SCSGSSGVGE ; AGEDLSYDTG ; 200: PGPAFKEVWQ ; VILKPKGLGQ ; TKNLIGIYRL ; CLTSKTISFV ; KLNSEAAAVV ; 250: LQLMNIRRCG ; HSENFFFIEV ; GRSAVTGPGE ; FWMQVDDSVV ; AQNMHETILE ; 300: AMRAMSDEFR ; PRTKSQSSSS ; CSNPISVPLR ; RHHLNNPPPS ; QVGLTRRSRT ; 350: ESITATSPAS ; MVGGKPGSFR ; VRASSDGEGT ; MSRPASVDGS ; PVSPSTNRTH ; 400: AHRHRGSSRL ; HPPLNHSRSI ; PMPSSRCSPS ; ATSPVSLSSS ; STSGHGSTSD ; 450: CLFPRRSSAS ; VSGSPSDGGF ; ISSDEYGSSP ; CDFRSSFRSV ; TPDSLGHTPP ; 500: ARGEEELSNY ; ICMGGKGAST ; LTAPNGHYIL ; SRGGNGHRYI ; PGATMGTSPA ; 550: LTGDEAAGAA ; DLDNRFRKRT ; HSAGTSPTIS ; HQKTPSQSSV ; VSIEEYTEMM ; 600: PAAYPPGGGS ; GGRLPGYRHS ; AFVPTHSYPE ; EGLEMHHLER ; RGGHHRPDSS ; 650: NLHTDDGYMP ; MSPGVAPVPS ; NRKGNGDYMP ; MSPKSVSAPQ ; QIINPIRRHP ; 700: QRVDPNGYMM ; MSPSGSCSPD ; IGGGSCSSSS ; ISAAPSGSSY ; GKPWTNGVGG ; 750: HHTHALPHAK ; PPVESGGGKL ; LPCTGDYMNM ; SPVGDSNTSS ; PSECYYGPED ; 800: PQHKPVLSYY ; SLPRSFKHTQ ; RPGEPEEGAR ; HQHLRLSSSS ; GRLRYTATAE ; 850: DSSSSTSSDS ; LGGGYCGARP ; ESSVTHPHHH ; ALQPHLPRKV ; DTAAQTNSRL ; 900: ARPTRLSLGD ; PKASTLPRVR ; EQQQQQQQQQ ; QSSLHPPEPK ; SPGEYVNIEF ; 950: GSGQPGYLAG ; PATSRSSPSV ; RCLPQLHPAP ; REETGSEEYM ; NMDLGPGRRA ; 1000: TWQESGGVEL ; GRVGPAPPGA ; ASICRPTRSV ; PNSRGDYMTM ; QIGCPRQSYV ; 1050: DTSPVAPVSY ; ADMRTGIAAE ; KVSLPRTTGA ; APPPSSTASA ; SASVTPQGAA ; 1100: EQAAHSSLLG ; GPQGPGGMSA ; FTRVNLSPNH ; NQSAKVIRAD ; TQGCRRRHSS ; 1150: ETFSAPTRAA ; NTVSFGAGAA ; GGGSGGGSED ; VKRHSSASFE ; NVWLRPGDLG ; 1200: GASKESAPGC ; GAAGGLEKSL ; NYIDLDLVKD ; VKQHPQDCPS ; QQQSLPPPPP ; 1235: HQPLGSNEGS ; SPRRSSEDLS ; TYASINFQKQ ; PEDRQ
Sequence Length	1235
String	P35570
Sub Unit	Interacts with SOCS7 (By similarity). Interacts (via IRS-type PTB domain) with IGF1R and INSR (via the tyrosine-phosphorylated NPXY motif) (By similarity). Interacts with UBTF, FER and PIK3CA (By similarity). Interacts (via phosphorylated YXXM motifs) with PIK3R1. Interacts with ROCK1. Interacts (via PH domain) with PHIP. Interacts with GRB2. Interacts with ALK.

Uniprot	P35570
Gene Name	Irs1
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:25467
Unigene	Rn.10476

Reference	1. "Structure of the insulin receptor substrate IRS-1 defines a unique signal transduction protein." Sun X.-J., Rothenberg P.L., Kahn C.R., Backer J.M., Araki E., Wilden P.A., Cahill D.A., Goldstein B.J., White M.F. Nature352:73-77(1991) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PARTIAL PROTEIN SEQUENCE strain: Sprague-Dawley. tissue: Liver. 2. "Purification and partial sequence analysis of pp185, the major cellular substrate of the insulin receptor tyrosine kinase." Rothenberg P.L., Lane W.S., Karasik A., Backer J.M., White M.F., Kahn C.R. J. Biol. Chem.266:8302-8311(1991) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 44-51; 173-178; 223-243; 489-506; 635-646; 932-947 AND 1098-1106 3. "Phosphatidylinositol 3'-kinase is activated by association with IRS-1 during insulin stimulation." Backer J.M., Myers M.G. Jr., Shoelson S.E., Chin D.J., Sun X.-J., Miralpeix M., Hu P., Margolis B., Skolnik E.Y., Schlessinger J., White M.F. EMBO J.11:3469-3479(1992) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;PHOSPHORYLATION;INTERACTION WITH PIK3R1 4. "The SH2/SH3 domain-containing protein GRB2 interacts with tyrosine-phosphorylated IRS1 and Shc: implications for insulin control of ras signalling." Skolnik E.Y., Lee C.-H., Batzer A.G., Vicentini L.M., Zhou M., Daly R.J., Myers M.J. Jr., Backer J.M., Ullrich A., White M.F., Schlessinger J. EMBO J.12:1929-1936(1993) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH GRB2 5. "Pleiotropic insulin signals are engaged by multisite phosphorylation of IRS-1." Sun X.-J., Crimmins D.L., Myers M.G. Jr., Miralpeix M., White M.F. Mol. Cell. Biol.13:7418-7428(1993) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT TYR-460; TYR-608; TYR-628; TYR-895; TYR-939; TYR-987; TYR-1172 AND TYR-1222 6. "Phosphorylation of the insulin receptor substrate IRS-1 by casein kinase II." Tanasijevic M.J., Myers M.G. Jr., Thoma R.S., Crimmins D.L., White M.F., Sacks D.B. J. Biol. Chem.268:18157-18166(1993) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-99 AND THR-502 7. "Cloning and characterization of PHIP, a novel insulin receptor substrate-1 pleckstrin homology domain interacting protein." Farhang-Fallah J., Yin X., Trentin G., Cheng A.M., Rozakis-Adcock M. J. Biol. Chem.275:40492-40497(2000) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH PHIP;MUTAGENESIS OF TRP-106 8. "Phosphorylation of Ser307 in insulin receptor substrate-1 blocks interactions with the insulin receptor and inhibits insulin action." Aguirre V., Werner E.D., Giraud J., Lee Y.H., Shoelson S.E., White M.F. J. Biol. Chem.277:1531-1537(2002) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-307 9. "In vivo phosphorylation of insulin receptor substrate 1 at serine 789 by a novel serine kinase in insulin-resistant rodents." Qiao L.Y., Zhande R., Jetton T.L., Zhou G., Sun X.-J. J. Biol. Chem.277:26530-26539(2002) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-789 10. "Active Rho kinase (ROK-alpha) associates with insulin receptor substrate-1 and inhibits insulin signaling in vascular smooth muscle cells." Begum N., Sandu O.A., Ito M., Lohmann S.M., Smolenski A. J. Biol. Chem.277:6214-6222(2002) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH ROCK1;PHOSPHORYLATION

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