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Home / Assay Kits / ELISA Kits

ELISA Kit for Rat Hypoxia-inducible factor 1-alpha

SKU : BHE13804628

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E0798r
Categories	Elisa
Function	Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP.
Specificity	Natural and recombinant Rat Hypoxia-inducible factor 1-alpha
Subcellular Location	Cytoplasm Nucleus Cytoplasmic in normoxia, nuclear translocation in response to hypoxia.
Detection Range	7.8-500pg/mL
Entryname	HIF1A_RAT
Method Type	Sandwich
Platform	PF11413
Protein Name	Hypoxia-inducible factor 1-alpha
Sensitivity	3.9pg/mL
Sequence	50: MEGAGGENEK ; KKMSSERRKE ; KSRDAARSRR ; SKESEVFYEL ; AHQLPLPHNV ; 100: SSHLDKASVM ; RLTISYLRVR ; KLLDAGDLDI ; EDEMKAQMNC ; FYLKAPDGFV ; 150: MVLTDDGDMI ; YISDNVNKYM ; GLTQFELTGH ; SVFDFTHPCD ; HEEMREMLTH ; 200: RNGPVRKGKE ; QNTQRSFFLR ; MKCTLTSRGR ; TMNIKSATWK ; VLHCTGHIHV ; 250: YDTSSNQPQC ; GYKKPPMTCL ; VLICEPIPHP ; SNIEIPLDSK ; TFLSRHSLDM ; 300: KFSYCDERIT ; ELMGYEPEEL ; LGRSIYEYYH ; ALDSDHLTKT ; HHDMFTKGQV ; 350: TTGQYRMLAK ; RGGYVWVETQ ; ATVIYNTKDS ; QPQCIVCVNY ; VVSGIIQHDL ; 400: IFSLQQTESV ; LKPVESSDMK ; MTQLFTKVES ; EDTSCLFDKL ; KKEPDALTLL ; 450: APAAGDTIIS ; LDFGSDDTET ; EDQQLEDVPL ; YNDVMFPSSN ; EKLNINLAMS ; 500: PLPASETPKP ; LRSSADPALN ; QEVALKLESS ; PESLGLSFTM ; PQIQDQPASP ; 550: SDGSTRQSSP ; EPNSPSEYCF ; DVDSDMVNVF ; KLELVEKLFA ; EDTEAKNPFS ; 600: AQDTDLDLEM ; LAPYIPMDDD ; FQLRSFDQLS ; PLESNSPSPP ; SVSTVTGFQQ ; 650: TQLQKPTITV ; TAATATTATT ; TDESKAVTKD ; NIEDIKILIA ; SPPSTQVPQE ; 700: MTTAKASAYS ; GTHSRTASPD ; RAGKRVIEKT ; DKAHPRSLNL ; SVTLNQRNTV ; 750: PEEELNPRTI ; ALQNAQRKRK ; MEHDGSLFQA ; AGIGTLLQQP ; GDRAPTMSLS ; 800: WKRVKGYISS ; EQDGMEQKTI ; FLIPSDLACR ; LLGQSMDESG ; LPQLTSYDCE ; 825: VNAPIQGSRN ; LLQGEELLRA ; LDQVN
Sequence Length	825
String	O35800
Sub Unit	Interacts with COPS5 subunit of COP9 signalosome complex, leading to the regulation of its stability. Efficient DNA binding requires heterodimerization of an alpha and a beta/ARNT subunit. Interacts with NCOA1, NCOA2, APEX, HSP90 and TSGA10. Interacts with VHL which docks HFA1 to the E3 ubiquitin ligase complex for subsequent destruction. Interaction, via the ODD domain, with the beta domain of VHLL, protects HIF1A from destruction by competing against the destructive targeting initiated by VHL. Interacts with RORA (via the DNA bi nding domain); the interaction enhances HIF1A transcription under hypoxia through increasing protein stability. Interaction with PSMA7 inhibits the transactivation activity of HIF1A under both normoxic and hypoxia-mimicking conditions (By similarity). Interacts with USP20. Interacts with GNB2L1/RACK1; promotes HIF1A ubiquitination and proteasome-mediated degradation Interacts with EP300 (via TAZ-type 1 domain); the interaction is stimulated in response to hypoxia and inhibited by CITED2. Interacts with CREBBP (via TAZ-type 1 domain).

Uniprot	O35800
Gene Name	Hif1a
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:29560
Unigene	Rn.10852

Reference	1. "Perivenous expression of the mRNA of the three hypoxia-inducible factor a-subunits HIF-1a, HIF2a and HIF3a in rat liver." Kietzmann T., Cornesse Y., Brechtel K., Modaressi S., Jungermann K. Biochem. J.354:531-537(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: Wistar. tissue: Hepatocyte. 2. "Oxygen-dependent expression of hypoxia-inducible factor-1alpha in renal medullary cells of rats." Zou A.-P., Yang Z.-Z., Li P.-L., Cowley A.W. Jr. Physiol. Genomics6:159-168(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: Sprague-Dawley. tissue: Kidney.

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BHE13804628	E0798r	96T	$796		Add to Cart

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