ELISA Kit for Rat H-2 class II histocompatibility antigen gamma chain

Mfr.No.

E13627r

Alternative Names

Ia antigen-associated invariant chain ; Ii ; MHC class II-associated invariant chain ; CD74

Categories

Elisa

Function

Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to compartments where peptide loading of class II takes place.

Specificity

Natural and recombinant Rat H-2 class II histocompatibility antigen gamma chain

Subcellular Location

Membrane Single-pass type II membrane protein

Detection Range

0.312-20ng/mL

Entryname

HG2A_RAT

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	89-99%	90-101%	93-101%	92-106%
EDTA plasma(n=5)	89-97%	90-102%	89-102%	90-106%
heparin plasma(n=5)	93-104%	94-108%	101-109%	93-110%

Method Type

Sandwich

Platform

PF09307

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 5.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 9.3%

Protein Name

H-2 class II histocompatibility antigen gamma chain

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	94-98
Plasma	97	93-99

Sensitivity

0.156ng/mL

Sequence

50:

MDDQRDLISN ; HEQLPILGQR ; ARAPESNCNR ; GVLYTSVSVL ; VALLLAGQAT ;

100:

TAYFLYQQQG ; RLDKLTVTSQ ; NLQLENLRMK ; LPKSAKPVSP ; MRMATPLLMR ;

150:

PLSMDNMLQA ; PVKNVTKYGN ; MTQDHVMHLL ; TKSGPVNYPQ ; LKGSFPENLK ;

200:

HLKNSMNGLD ; WKVFESWMKQ ; WLLFEMSKNS ; LEEKQPTQTP ; PKVLTKCQEE ;

250:

VSHIPDVHPG ; AFRPKCDENG ; NYMPLQCHGS ; TGYCWCVFPN ; GTEVPHTKSR ;

280:

GRHNCSEPLD ; MEDPSSGLGV ; TKQDMGQMFL ;

Sequence Length

280

String

P10247

Sub Unit

Nonamer composed of three alpha/beta/gamma heterotrimers.

Uniprot	P10247
Gene Name	Cd74
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:25599
Unigene	Rn.33804

Reference	1. "Sequence of a rat MHC class II-associated invariant chain cDNA clone containing a 64 amino acid thyroglobulin-like domain." McKnight A.J., Mason D.W., Barclay A.N. Nucleic Acids Res.17:3983-3984(1989) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: PVG X DA. tissue: Spleen. 2. "Nucleotide sequence of rat invariant gamma chain cDNA clone pLR gamma 34.3." Henkes W., Syha J., Reske K. Nucleic Acids Res.16:11822-11822(1988) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-192 AND 257-280 strain: Lewis.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13804979	E13627r	96T	$796		Add to Cart

ELISA Kit for Rat H-2 class II histocompatibility antigen gamma chain

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