ELISA Kit for Rat Granzyme B

Mfr.No.

E0600r

Alternative Names

Fragmentin ; Natural killer cell protease 1 ; RNKP-1

Categories

Elisa

Function

This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

Specificity

Natural and recombinant Rat Granzyme B

Detection Range

15.6-1000pg/mL

Entryname

GRAB_RAT

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Granzyme B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	80-96%	82-10%	87-98%	96-103%
EDTA plasma(n=5)	90-105%	93-102%	86-105%	84-102%
heparin plasma(n=5)	85-99%	85-94%	84-101%	85-101%

Method Type

Sandwich

Platform

PF00089

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.1

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.9

Protein Name

Granzyme B

Recovery

Recovery was determined by spiking various levels of Granzyme B into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	101	85-109
Plasma	99	94-110

Sensitivity

7.86pg/mL

Sequence

50:

MKLLLLLLSF ; SLAPKTEAGE ; IIGGHEAKPH ; SRPYMAYLQI ; MDEYSGSKKC ;

100:

GGFLIREDFV ; LTAAHCSGSK ; INVTLGAHNI ; KEQEKMQQII ; PVVKIIPHPA ;

150:

YNSKTISNDI ; MLLKLKSKAK ; RSSAVKPLNL ; PRRNVKVKPG ; DVCYVAGWGK ;

200:

LGPMGKYSDT ; LQEVELTVQE ; DQKCESYLKN ; YFDKANEICA ; GDPKIKRASF ;

248:

RGDSGGPLVC ; KKVAAGIVSY ; GQNDGSTPRA ; FTKVSTFLSW ; IKKTMKKS

Sequence Length

248

String

P18291

Uniprot	P18291
Gene Name	Gzmb
Immunogen Species	Rattus norvegicus (Rat)
Kegg	o:171528
Unigene	Rn.132115

Reference	1. "RNKP-1, a novel natural killer-associated serine protease gene cloned from RNK-16 cytotoxic lymphocytes." Zunino S.J., Bleackley R.C., Martinez J., Hudig D. J. Immunol.144:2001-2009(1990) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: T-cell. 2. "Purification of a factor from the granules of a rat natural killer cell line (RNK) that reduces tumor cell growth and changes tumor morphology. Molecular identity with a granule serine protease (RNKP-1)." Sayers T.J., Wiltrout T.A., Sowder R., Munger W.L., Smyth M.J., Henderson L.E. J. Immunol.148:292-300(1992) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 21-53 3. "A natural killer cell granule protein that induces DNA fragmentation and apoptosis." Shi L., Kraut R.P., Aebersold R., Greenberg A.H. J. Exp. Med.175:553-566(1992) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PARTIAL PROTEIN SEQUENCE 4. "The structure of the pro-apoptotic protease granzyme B reveals the molecular determinants of its specificity." Waugh S.M., Harris J.L., Fletterick R., Craik C.S. Nat. Struct. Biol.7:762-765(2000) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 21-248 IN COMPLEX WITH E.COLI ECOTIN;ACTIVE SITE;DISULFIDE BONDS

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ELISA Kit for Rat Granzyme B

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