ELISA Kit for Rat E-selectin

Mfr.No.

E0029r

Alternative Names

CD62 antigen-like family member E ; Endothelial leukocyte adhesion molecule 1 ; ELAM-1 ; Leukocyte-endothelial cell adhesion molecule 2 ; LECAM2 ; CD62E ; Elam-1

Categories

Elisa

Function

Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.

Specificity

Natural and recombinant Rat E-selectin

Subcellular Location

Membrane Single-pass type I membrane protein

Detection Range

0.156-10ng/mL

Entryname

LYAM2_RAT

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ELAM1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-95%	85-89%	95-100%	93-98%
EDTA plasma(n=5)	98-105%	99-104%	92-96%	91-98%
heparin plasma(n=5)	89-93%	91-96%	95-104%	98-107%

Method Type

Sandwich

Platform

PF00008

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.1%

Protein Name

E-selectin

Recovery

Recovery was determined by spiking various levels of ELAM1into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	95-104
Plasma	98	93-99

Sensitivity

0.092ng/mL

Sequence

50:

MNASCFLSAL ; TFVLLIGKSI ; AWYYNASSEL ; MTYDEASAYC ; QRDYTHLVAI ;

100:

QNKEEINYLN ; STLRYSPSYY ; WIGIRKVNNV ; WIWVGTQKPL ; TEEAKNWAPG ;

150:

EPNNKQRNED ; CVEIYIQRPK ; DSGMWNDERC ; DKKKLALCYT ; ASCTNTSCSG ;

200:

HGECVETINS ; YTCKCHPGFL ; GPKCDQVVTC ; QEQEYPDHGS ; LNCTHPFGLF ;

250:

SYNSSCSFSC ; ERGYVPSSME ; TTVRCTSSGE ; WSAPAPACHV ; VECKALTQPA ;

300:

HGVRKCSSNP ; GSYPWNTTCT ; FDCEEGYRRV ; GAQNLQCTSS ; GVWDNEKPSC ;

350:

KAVTCDAIPR ; PQNGSVSCSN ; STAGALAFKS ; SCNFTCEHSF ; TLQGPAQVEC ;

400:

SAQGQWTPQI ; PVCKASQCEA ; LSAPQRGHMK ; CLPSASAPFQ ; SGSSCKFSCD ;

450:

EGFELKGSRR ; LQCGPRGEWD ; SEKPTCAGVQ ; CSSLDLPGKM ; NMSCSGPAVF ;

500:

GTVCEFTCPE ; GWTLNGSSIL ; TCGATGRWSA ; MLPTCEAPAN ; PPRPLVVALS ;

549:

VAATSLLTLS ; SLIYVLKRFF ; WKKAKKFVPA ; SSCQSLQSFE ; NYQGPSYII

Sequence Length

549

String

P98105

Sub Unit

Interacts with PSGL1/SELPLG and PODXL2 through the sialyl Lewis X epitope. PSGL1 sulfation appears not to be required for this interaction.

Uniprot	P98105
Gene Name	Sele
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:25544
Unigene	Rn.10359

Reference	1. Rosenbloom C.L., Auchampach J.A., Anderson D.C., Manning A.M. Submitted (1993-11) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Lung.

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ELISA Kit for Rat E-selectin

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