ELISA Kit for Rat DnaJ homolog subfamily C member 27

Mfr.No.

E3713r

Alternative Names

Rab and DnaJ domain-containing protein ; Rbj

Categories

Elisa

Specificity

Natural and recombinant Rat DnaJ homolog subfamily C member 27

Detection Range

0.156-10ng/mL

Entryname

DJC27_RAT

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DJC27 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	84-100%	90-96%	85-98%	89-95%
EDTA plasma(n=5)	88-101%	87-102%	90-97%	85-101%
heparin plasma(n=5)	90-99%	86-101%	91-98%	84-103%

Method Type

Sandwich

Platform

PF00226

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 6.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 9.4%

Protein Name

DnaJ homolog subfamily C member 27

Recovery

Recovery was determined by spiking various levels of DJC27 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	92	85-96
Plasma	94	87-102

Sensitivity

0.083ng/mL

Sequence

50:

MESNVPKRKE ; PLKSLRIKVI ; SMGNAEVGKS ; CIIKRYCEKR ; FVSKYLATIG ;

100:

IDYGVTKVQV ; RDREIKVNIF ; DMAGHPFFFE ; VRNEFYKDTQ ; GVILVYDVGQ ;

150:

KDSFDALDSW ; LAEMKQELGP ; HGNMENIVFV ; VCANKIDCSK ; HRCIDESEGR ;

200:

LWAESRGFLY ; FETSAQTGEG ; INEMFQTFYM ; SIVDLCENGG ; KRPTANSSAS ;

250:

YTKEQADTIR ; RIRTSKDSWE ; MLGVRPGASR ; EEVNKAYRKL ; AVLLHPDKCV ;

273:

APGSEDAFKA ; VVNARTALLK ; NIK

Sequence Length

273

String

Q6IML7

Uniprot	Q6IML7
Gene Name	Dnajc27
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:298859
Unigene	Rn.22692

Reference	1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-169 tissue: Brain. 2. "RJLs: a new family of Ras-related GTP-binding proteins." Nepomuceno-Silva J.L., de Melo L.D., Mendonca S.M., Paixao J.C., Lopes U.G. Gene327:221-232(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION

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BHE13803737	E3713r	96T	$796		Add to Cart

ELISA Kit for Rat DnaJ homolog subfamily C member 27

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