ELISA Kit for Rat Decorin

Mfr.No.

E6238r

Alternative Names

Bone proteoglycan II ; Dermatan sulfate proteoglycan-II ; DSPG ; PG-S2 ; PG40

Categories

Elisa

Function

May affect the rate of fibrils formation (By similarity). May be implicated in the dilatation of the rat cervix.

Specificity

Natural and recombinant Rat Decorin

Subcellular Location

Secreted extracellular space extracellular matrix

Detection Range

31.2-2000pg/mL

Entryname

PGS2_RAT

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PGS2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-110%	98-107%	95-109%	99-108%
Saliva(n=3)	94-108%	93-109%	97-106%	95-107%
heparin plasma(n=5)	93-111%	95-105%	94-110%	98-106%

Method Type

Sandwich

Platform

PF00560

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.1%

Protein Name

Decorin

Recovery

Recovery was determined by spiking various levels of PGS2 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	103	94-105
Plasma	101	93-106

Sensitivity

14.4pg/mL

Sequence

50:

MKATLVLFLL ; AQVSWAGPFE ; QRGLFDFMLE ; DEASGIIPYD ; PDNPLISMCP ;

100:

YRCQCHLRVV ; QCSDLGLDKV ; PWEFPPDTTL ; LDLQNNKITE ; IKEGAFKNLK ;

150:

DLHTLILVNN ; KISKISPEAF ; KPLVKLERLY ; LSKNHLKELP ; EKLPKTLQEL ;

200:

RLHDNEITKL ; KKSVFNGLNR ; MIVIELGGNP ; LKNSGIENGA ; LQGMKGLGYI ;

250:

RISDTNITAI ; PQGLPTSISE ; LHLDGNKIAK ; VDAASLKGMS ; NLSKLGLSFN ;

300:

SITVVENGSL ; ANVPHLRELH ; LDNNKLLRVP ; AGLAQHKYVQ ; VVYLHNNNIS ;

350:

EVGQHDFCLP ; SYQTRKTSYT ; AVSLYSNPVR ; YWQIHPHTFR ; CVFGRSTIQL ;

354:

GNYK

Sequence Length

354

String

Q01129

Sub Unit

Binds to type I and type II collagen, fibronectin and TGF-beta. Forms a teary complex with MFAP2 and ELN. Interacts with DPT.

Uniprot	Q01129
Gene Name	Dcn
Immunogen Species	Rattus norvegicus (Rat)
Kegg	o:29139
Unigene	Rn.106103

Reference	1. "cDNA sequence for rat dermatan sulfate proteoglycan-II (decorin)." Abramson S.R., Woessner J.F. Biochim. Biophys. Acta1132:225-227(1992) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: Sprague-Dawley. tissue: Uterus. 2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] tissue: Heart. 3. "Molecular characterization of vascular smooth muscle decorin: deduced core protein structure and regulation of gene expression." Asundi V.K., Dreher K.L. Eur. J. Cell Biol.59:314-321(1992) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-354 4. "Purification and characterization of a small dermatan sulphate proteoglycan implicated in the dilatation of the rat uterine cervix." Kokenyesi R., Woessner J.F. Biochem. J.260:413-419(1989) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 31-48 AND 171-191 strain: Sprague-Dawley. tissue: Uterus.

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE13809360	E6238r	96T	$796		Add to Cart

ELISA Kit for Rat Decorin

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of ELISA Kit for Rat Decorin

Rate it now..!!