ELISA Kit for Rat Bone morphogenetic protein 4

Mfr.No.

E0014r

Alternative Names

Bone morphogenetic protein 2B ; BMP-2B ; Bmp-4 ; Dvr-4

Categories

Elisa

Function

Induces cartilage and bone formation. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.

Specificity

Natural and recombinant Rat Bone morphogenetic protein 4

Subcellular Location

Secreted extracellular space extracellular matrix

Detection Range

78-5000pg/mL

Entryname

BMP4_RAT

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BMP4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-100%	98-101%	94-99%	96-100%
EDTA plasma(n=5)	95-99%	98-103%	100-103%	102-106%
heparin plasma(n=5)	96-101%	98-100%	95-103%	101-105%

Method Type

Sandwich

Platform

PF00019

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.9%

Protein Name

Bone morphogenetic protein 4

Recovery

Recovery was determined by spiking various levels of BMP4 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	96-102
Plasma	98	94-100

Sensitivity

39pg/mL

Sequence

50:

MIPGNRMLMV ; VLLCQVLLGG ; ATDASLIPET ; GKKKVAEIQG ; HAGGRRSGQS ;

100:

HELLRDFEAT ; LLQMFGLRRR ; PQPSKSAVIP ; DYMRDLYRLQ ; SGEEEEEEQS ;

150:

QGTGLEYPER ; PASRANTVRS ; FHHEEHLENI ; PGTSESSAFR ; FFFNLSSIPE ;

200:

NEVISSAELR ; LFREQVDQGP ; DWEQGFHRIN ; IYEVMKPPAE ; MVPGHLITRL ;

250:

LDTRLVRHNV ; TRWETFDVSP ; AVLRWTREKQ ; PNYGLAIEVT ; HLHQTRTHQG ;

300:

QHVRISRSLP ; QGSGNWAQLR ; PLLVTFGHDG ; RGHTLTRRRA ; KRSPKHHPQR ;

350:

SRKKNKNCRR ; HSLYVDFSDV ; GWNDWIVAPP ; GYQAFYCHGD ; CPFPLADHLN ;

400:

STNHAIVQTL ; VNSVNSSIPK ; ACCVPTELSA ; ISMLYLDEYD ; KVVLKNYQEM ;

408:

VVEGCGCR

Sequence Length

408

String

Q06826

Sub Unit

Homodimer; disulfide-linked. Interacts with SOSTDC1, GREM2, RGMA, RGMB and RGMC (By similarity). Part of a complex consisting of TWSG1 and CHRD.

Uniprot	Q06826
Gene Name	Bmp4
Immunogen Species	Rattus norvegicus (Rat)
Unigene	Rn.10318

Reference	1. "Cloning and sequence of bone morphogenetic protein 4 cDNA from fetal rat calvarial cell." Chen D., Feng J.Q., Feng M., Harris M.A., Mundy G.R., Harris S.E. Biochim. Biophys. Acta1174:289-292(1993) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]

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BHE13812083	E0014r	96T	$796		Add to Cart

ELISA Kit for Rat Bone morphogenetic protein 4

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