ELISA Kit for Rat Bone morphogenetic protein 2

Mfr.No.

E0013r

Alternative Names

Bone morphogenetic protein 2A ; BMP-2A ; Bmp-2

Categories

Elisa

Function

Induces cartilage and bone formation.

Specificity

Natural and recombinant Rat Bone morphogenetic protein 2

Subcellular Location

Secreted

Detection Range

62.5-4000pg/mL

Entryname

BMP2_RAT

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BMP2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	101-105%	96-101%	99-105%	103-106%
EDTA plasma(n=5)	99-105%	102-104%	95-99%	98-104%
heparin plasma(n=5)	100-106%	92-97%	101-104%	106-110%

Method Type

Sandwich

Platform

PF00019

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.6%

Protein Name

Bone morphogenetic protein 2

Recovery

Recovery was determined by spiking various levels of BMP2 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	101	98-103
Plasma	100	99-106

Sensitivity

31.25pg/mL

Sequence

50:

MVAGTRCLLV ; LLLPQVLLGG ; AAGLIPELGR ; KKFAGASRPL ; SRPSEDVLSE ;

100:

FELRLLSMFG ; LKQRPTPSKD ; VVVPPYMLDL ; YRRHSGQPGA ; LAPDHRLERA ;

150:

ASRANTVLSF ; HHEEAIEELS ; EMSGKTSRRF ; FFNLSSVPTD ; EFLTSAELQI ;

200:

FREQMQEALG ; NSSFQHRINI ; YEIIKPATAS ; SKFPVTRLLD ; TRLVTQNTSQ ;

250:

WESFDVTPAV ; MRWTAQGHTN ; HGFVVEVAHL ; EEKPGVSKRH ; VRISRSLHQD ;

300:

EHSWSQVRPL ; LVTFGHDGKG ; HPLHKREKRQ ; AKHKQRKRLK ; SSCKRHPLYV ;

350:

DFSDVGWNDW ; IVAPPGYHAF ; YCHGECPFPL ; ADHLNSTNHA ; IVQTLVNSVN ;

393:

SKIPKACCVP ; TELSAISMLY ; LDENEKVVLK ; NYQDMVVEGC ; GCR

Sequence Length

393

String

P49001

Sub Unit

Homodimer; disulfide-linked. Interacts with SOSTDC1 and GREM2. Interacts with ASPN.

Uniprot	P49001
Gene Name	Bmp2
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:29373
Unigene	Rn.90931

Reference	1. Feng J.Q., Chen D., Feng M., Harris M.A., Mundy G.R., Harris S.E. Submitted (1993-09) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Bone.

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BHE13801398	E0013r	96T	$796		Add to Cart

ELISA Kit for Rat Bone morphogenetic protein 2

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