ELISA Kit for Rat Beta-2 adrenergic receptor

Mfr.No.

E9974r

Alternative Names

Beta-2 adrenoreceptor ; Beta-2 adrenoceptor ; Adrb2r

Categories

Elisa

Function

Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.

Specificity

Natural and recombinant Rat Beta-2 adrenergic receptor

Subcellular Location

Cell membrane Multi-pass membrane protein

Detection Range

0.78-50ng/ml

Entryname

ADRB2_RAT

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ADRB2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	97-105%	95-108%	95-110%	96-109%
Saliva(n=3)	96-106%	95-105%	95-107%	93-107%
heparin plasma(n=5)	97-103%	93-108%	92-108%	94-110%

Method Type

Sandwich

Platform

PF00001

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.3%

Protein Name

Beta-2 adrenergic receptor

Recovery

Recovery was determined by spiking various levels of ADRB2 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	102	97-105
Plasma	101	96-106

Sensitivity

0.42ng/mL

Sequence

50:

MEPHGNDSDF ; LLAPNGSRAP ; GHDITQERDE ; AWVVGMAILM ; SVIVLAIVFG ;

100:

NVLVITAIAK ; FERLQTVTNY ; FITSLACADL ; VMGLAVVPFG ; ASHILMKMWN ;

150:

FGNFWCEFWT ; SIDVLCVTAS ; IETLCVIAVD ; RYVAITSPFK ; YQSLLTKNKA ;

200:

RVVILMVWIV ; SGLTSFLPIQ ; MHWYRATHKQ ; AIDCYAKETC ; CDFFTNQAYA ;

250:

IASSIVSFYV ; PLVVMVFVYS ; RVFQVAKRQL ; QKIDKSEGRF ; HAQNLSQVEQ ;

300:

DGRSGHGLRS ; SSKFCLKEHK ; ALKTLGIIMG ; TFTLCWLPFF ; IVNIVHVIRA ;

350:

NLIPKEVYIL ; LNWLGYVNSA ; FNPLIYCRSP ; DFRIAFQELL ; CLRRSSSKTY ;

400:

GNGYSSNSNG ; RTDYTGEQSA ; YQLGQEKENE ; LLCEEAPGME ; GFVNCQGTVP ;

418:

SLSIDSQGRN ; CNTNDSPL

Sequence Length

418

String

P10608

Sub Unit

Binds SLC9A3R1 and GPRASP1. Interacts with ARRB1 and ARRB2. Interacts with SRC, USP20 and USP33.

Uniprot	P10608
Gene Name	Adrb2
Immunogen Species	Rattus norvegicus (Rat)
Unigene	Rn.10206

Reference	1. "Primary structure of rat cardiac beta-adrenergic and muscarinic cholinergic receptors obtained by automated DNA sequence analysis: further evidence for a multigene family." Gocayne J.D., Robinson D.A., Fitzgerald M.G., Chung F.-Z., Kerlavage A.R., Lentes K.-U., Lai J., Wang C.-D., Fraser C.M., Venter J.C. Proc. Natl. Acad. Sci. U.S.A.84:8296-8300(1987) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] 2. "Primary structure of the rat beta-2 adrenergic receptor gene." Buckland P.R., Hill R.M., Tidmarsh S.F., McGuffin P. Nucleic Acids Res.18:682-682(1990) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] tissue: Liver. 3. "Sequence of the 5' regulatory domain of the gene encoding the rat beta 2-adrenergic receptor." Jiang L., Kunos G. Gene163:331-332(1995) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] strain: Sprague-Dawley. 4. "Structural and functional analysis of the 5'-flanking region of the rat beta 2-adrenergic receptor gene." McGraw D.W., Jacobi S.E., Hiller F.C., Cornett L.E. Biochim. Biophys. Acta1305:135-138(1996) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23

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ELISA Kit for Rat Beta-2 adrenergic receptor

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