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Home / Assay Kits / ELISA Kits

ELISA Kit for Rat Apoptotic protease-activating factor 1

SKU : BHE13801260

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E1054r
Categories	Elisa
Function	Regulates progammed cell death; necessary for normal brain development. Participates with caspase-9 (Apaf-3) in the cytochrome c-dependent activation of caspase-3, leading to apoptosis. This activation requires ATP.
Specificity	Natural and recombinant Rat Apoptotic protease-activating factor 1
Subcellular Location	Cytoplasm
Detection Range	0.312-20ng/mL
Entryname	APAF_RAT
Method Type	Sandwich
Platform	PF00619
Protein Name	Apoptotic protease-activating factor 1
Sensitivity	0.161ng/mL
Sequence	50: MDAKARNCLL ; QHKEALEKDI ; KTSYIMDHMI ; SNGVLTVVEE ; EKVKSQATQY ; 100: QRAAALIKMI ; LNKDNYAYIS ; FYNALLHEGY ; KDLAGLLHSG ; LPLVSSSSGK ; 150: DTDGGNTSFV ; RTVLCEGGVP ; QRPVIFVTRK ; KLVSAIQQKL ; WKLNGEPGWV ; 200: TIYGMAGCGK ; SVLAAEAVRD ; HALLEGCFSG ; GVHWVSIGKQ ; DKSGLLMKLQ ; 250: NLCTRLGQEE ; SFSQRLPLNI ; EEAKDRLRVL ; MLRKHPRSLL ; ILDDVWDPWV ; 300: LKAFDNQCQI ; LLTTRDKSVT ; DSVMGPKYVI ; PVESGLGKEK ; GLEILSLFVN ; 350: MKKEDLPVEA ; HSIIKECKGS ; PLVVSLVGAL ; LRDFPNRWAY ; YLRQLQNKQF ; 400: KRIRKSSSYD ; YEALDEAMSI ; SVEMLREDIK ; DYYTDLSILQ ; KDVKVPTKVL ; 450: CVLWDLETEE ; VEDILQEFVN ; KSLLFCNRNG ; KSFCYYLHDL ; QVDFLTEKNR ; 500: SQLQDLHRKM ; VTQFQRYHQP ; HTLSPGQEDC ; MYWYNFLAYH ; MASAGMHKEL ; 550: CALMFSLDWI ; KAKTELVGPA ; HLIHEFVEYR ; HILDEKDCAV ; CENFQEFLSL ; 600: NGHLLGRQPF ; PNIVQLGLCE ; PETSEVYQQA ; KLQAKQEVDT ; GRLYLEWINK ; 650: KTIKNLSRLV ; VRPHTDAVYH ; ACFSQDGQRI ; ASCGADKTLQ ; VFKAETGEKL ; 700: LDIKAHEDEV ; LCCAFSSDDS ; YIATCSVDKK ; VKIWDSGTGK ; LVHTYEEHSE ; 750: QVNCCHFTNK ; SNHLLLATGS ; NDSFLKLWDL ; NQKECRNTMF ; GHTNSVTHCR ; 800: FSPDDELLAS ; CSADGTLKLW ; DVRSANEKKS ; INVKRFFLSS ; EDPPEDVEVI ; 850: VKCCSWSADG ; DRIIVAAKNK ; VLLLDIHTSG ; LLTEIHTGHH ; STIQYCDFSP ; 900: YDHLAVIALS ; QYCVELWNID ; SRVKVADCRG ; HLSWVHGVMF ; SPDGSSFLTA ; 950: SDDQTIRVWE ; TRKVCKNSAI ; VLKQEIDVVF ; QENEMMVLAV ; DNIRGLQLIA ; 1000: GKTGQIDYLP ; EAQVSCCCLS ; PHLEYVAFGD ; EEGAIKIIEL ; PNNRVFSSGI ; 1050: GHKKAVRHIQ ; FTADGKTLIS ; SSEDSVIQVW ; NWQTEEYVFL ; QAHQETVKDF ; 1100: RLLRDSRLLS ; WSFDGTVKVW ; NVITGRIERD ; FTCHQGTVLS ; CAISSDATKF ; 1150: SSTSADKTAK ; IWSFELPSPL ; HELKGHNSCV ; RCSAFSLDGI ; LLATGDDNGE ; 1200: IRIWNVSDGQ ; LLHLCAPISI ; EEGTATHGGW ; VTDVCFSPDR ; KMLVSAGGYL ; 1249: KWWNVVTGES ; SQTFYTNGTN ; LKKIHVSPDF ; RTYVTVDNLG ; ILYILQVLE
Sequence Length	1249
String	Q9EPV5
Sub Unit	Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains. Interacts with UACA. Interacts with APIP.

Uniprot	Q9EPV5
Gene Name	Apaf1
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:78963
Unigene	Rn.64522

Reference	1. Itoh T., Itoh A., Pleasure D. Submitted (2000-11) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: Sprague-Dawley. 2. "Differential expression of apoptotic protease-activating factor-1 and caspase-3 genes and susceptibility to apoptosis during brain development and after traumatic brain injury." Yakovlev A.G., Ota K., Wang G., Movsesyan V., Bao W.-L., Yoshihara K., Faden A.I. J. Neurosci.21:7439-7446(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DEVELOPMENTAL STAGE;INDUCTION BY BRAIN INJURY

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