ELISA Kit for Rat Aminopeptidase O

Mfr.No.

E12538r

Alternative Names

Onpep

Categories

Elisa

Function

Aminopeptidases catalyze the hydrolysis of amino acid residues from the N-terminus of peptide or protein substrates. Able to cleave angiotensin III to generate angiotensin IV, a bioactive peptide of the renin-angiotensin pathway. Not able to cleave angiotensin I and angiotensin II. May play a role in the proteolytic processing of bioactive peptides in tissues such as testis and heart.

Specificity

Natural and recombinant Rat Aminopeptidase O

Subcellular Location

Cytoplasm

Detection Range

78-5000pg/mL

Entryname

AMPO_RAT

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AMPO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	98-107%	95-111%	97-109%	94-110%
EDTA plasma(n=5)	93-108%	97-105%	95-107%	96-106%
heparin plasma(n=5)	96-104%	94-109%	98-105%	97-107%

Method Type

Sandwich

Platform

PF09127

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.1%

Protein Name

Aminopeptidase O

Recovery

Recovery was determined by spiking various levels of AMPO into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	103	96-105
Plasma	101	92-106

Sensitivity

40.2pg/mL

Sequence

50:

MDINLDPSRD ; DLPLMANTSH ; LLVKHYVLDL ; DVDFGSRVLE ; GNIVLFFGDG ;

100:

NRCKKQSSSS ; QETFQMESEE ; AYILRTAEPC ; HVPKMDSNTF ; SPKMGHREFA ;

150:

VFGKSDQDAF ; DNDGNHDNKE ; HDSESSSSKY ; CCDTGNHGRE ; DFLLVLDCCD ;

200:

LSVLKVEEVD ; VAAVPDLEKF ; TKAPNLTAAP ; EKRRCEIVRD ; LVALPADAWR ;

250:

EQLDCYTRCS ; QAPGCGELLF ; DSDKWSLQIR ; KKGVPTAADF ; PHAIRIWYKT ;

300:

KPEGQSVTWT ; SDQDGRPCVY ; TMGSPINNRA ; LFPCQEPPVA ; MSTWQATVRA ;

350:

AASFVVLMSG ; EKSAKPTPLR ; ESWHYYVTMP ; MPASTFTIAV ; GCWTEMKPKT ;

400:

SPLDDLTEHT ; LPLRPSDADF ; RFVFGNCSHM ; EYPCRFQSAS ; AATQNIIPYR ;

450:

VFAPVCLEGA ; CREALLWLIP ; SCLSAAHSVL ; GTHPFSRLDI ; LIVPTNFPSL ;

500:

GMASPHIIFL ; SQSTLTGTSH ; LCGTRLCHEI ; AHAWFGLAIG ; ARDWTEEWLS ;

550:

EGFATHLEDI ; FWAEAQQLPP ; HEALEQQELR ; ACLRWHRLQD ; ELQNSPEGMQ ;

600:

VLRPNKEKTG ; HVSASGASVV ; KNGLNPEKGF ; MQVHYLKGYF ; LLRFLARTLG ;

650:

EETYFPFLRK ; FVHLFHGQLI ; LSQDFLQMLL ; ESIPENKRFG ; LSVENIVGDW ;

700:

LECPGIPKAL ; QEERKAKDCS ; PSRLVRQVAK ; WIRVNRRPGK ; RQRRKREAAF ;

750:

EKLSPDQIVL ; LLEWLLEQKT ; LSPQTLHRLQ ; QTYHLQEQDA ; EVRHRWCELV ;

800:

IKHKYTKAYD ; QVKRFLQEDQ ; AMGIYLYGEL ; MVSEDARLQQ ; LAHRCFELVK ;

816:

GHMDKASAQV ; VTEMLF

Sequence Length

816

String

P69527

Uniprot	P69527
Gene Name	Aopep
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:290963
Unigene	Rn.263

Reference	1. "Identification of human aminopeptidase O, a novel metalloprotease with structural similarity to aminopeptidase B and leukotriene A4 hydrolase." Diaz-Perales A., Quesada V., Sanchez L.M., Ugalde A.P., Suarez M.F., Fueyo A., Lopez-Otin C. J. Biol. Chem.280:14310-14317(2005) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: Wistar. tissue: Lung.

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BHE13800696	E12538r	96T	$796		Add to Cart

ELISA Kit for Rat Aminopeptidase O

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