ELISA Kit for Pig Vascular endothelial growth factor A

Mfr.No.

E0143p

Alternative Names

Vascular permeability factor ; VPF ; VEGF

Categories

Elisa

Function

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin.

Specificity

Natural and recombinant Pig Vascular endothelial growth factor A

Subcellular Location

Secreted Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.

Detection Range

31.2-2000pg/mL

Entryname

VEGFA_PIG

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGF-A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	84-93%	79-94%	88-96%	78-91%
EDTA plasma(n=5)	78-89%	82-97%	90-102%	89-103%
heparin plasma(n=5)	83-94%	89-101%	87-99%	91-103%

Method Type

Sandwich

Platform

PF00341

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 4.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 10.2%

Protein Name

Vascular endothelial growth factor A

Recovery

Recovery was determined by spiking various levels of VEGF-A into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	86	80-95
Plasma	95	90-102

Sensitivity

8.3pg/mL

Sequence

50:

MNFLLSWVHW ; SLALLLYLHH ; AKWSQAAPMA ; EGDQKPHEVV ; KFMDVYQRSY ;

100:

CRPIETLVDI ; FQEYPDEIEY ; IFKPSCVPLM ; RCGGCCNDEG ; LECVPTEEFN ;

150:

ITMQIMRIKP ; HQGQHIGEMS ; FLQHNKCECR ; PKKDRARQEN ; PCGPCSERRK ;

190:

HLFVQDPQTC ; KCSCKNTDSR ; CKARQLELNE ; RTCRCDKPRR ;

Sequence Length

190

String

P49151

Sub Unit

Homodimer; disulfide-linked. Also found as heterodimer with PGF.

Uniprot	P49151
Gene Name	VEGFA
Immunogen Species	Sus Scrofa (Pig)
Kegg	ssc:397157
Unigene	Ssc.57541

Reference	1. "Nucleotide sequence and expression of the porcine vascular endothelial growth factor." Sharma H.S., Tang Z.H., Gho B.C.H., Verdouw P.D. Biochim. Biophys. Acta1260:235-238(1995) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Heart. 2. "PCR cloning of porcine cardiac vascular endothelial growth factor gene." Lee T., Canty J.M. Submitted (2000-11) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]

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SKU	Supplier No.	Size	Your price	Quantity
BHE13811393	E0143p	96T	$900		Add to Cart

ELISA Kit for Pig Vascular endothelial growth factor A

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