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ELISA Kit for Pig Serine/arginine-rich splicing factor 1

SKU : BHE13810708

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E8282p
Alternative Names	Splicing factor ; arginine/serine-rich 1 ; SFRS1
Categories	Elisa
Function	Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing.
Specificity	Natural and recombinant Pig Serine/arginine-rich splicing factor 1
Subcellular Location	Cytoplasm Nucleus Nucleus speckle Shuttles between the nucleus and the cytoplasm.
Detection Range	0.312-20ng/ml
Entryname	SRSF1_PIG
Method Type	Sandwich
Platform	PF00076
Protein Name	Serine/arginine-rich splicing factor 1
Sensitivity	0.083ng/ml
Sequence	50: MSGGGVIRGP ; AGNNDCRIYV ; GNLPPDIRTK ; DIEDVFYKYG ; AIRDIDLKNR ; 100: RGGPPFAFVE ; FEDPRDAEDA ; VYGRDGYDYD ; GYRLRVEFPR ; SGRGTGRGGG ; 150: GGGGGGAPRG ; RYGPPSRRSE ; NRVVVSGLPP ; SGSWQDLKDH ; MREAGDVCYA ; 200: DVYRDGTGVV ; EFVRKEDMTY ; AVRKLDNTKF ; RSHEGETAYI ; RVKVDGPRSP ; 248: SYGRSRSRSR ; SRSRSRSRSN ; SRSRSYSPRR ; SRGSPRYSPR ; HSRSRSRT
Sequence Length	248
String	Q3YLA6
Sub Unit	Consists of two polypeptides of p32 and p33. In vitro, self-associates and binds SRSF2, SNRNP70 and U2AF1 but not U2AF2. Binds SREK1/SFRS12. Interacts with SAFB/SAFB1. Interacts with PSIP1/LEDGF. Interacts with SRPK1. Identified in the spliceosome C complex. Interacts with RSRC1 (via Arg/Ser-rich domain). Interacts with ZRSR2/U2AF1-RS2. Interacts with CCDC55 (via C-terminus).

Uniprot	Q3YLA6
Gene Name	SRSF1
Immunogen Species	Sus Scrofa (Pig)
Kegg	ssc:654327
Unigene	Ssc.94271

Reference	1. "Molecular cloning, polymorphism and mapping of the porcine SFRS1 gene." Wang H., Wang H.L., Zhu Z.M., Yang S.L., Li K. Anim. Genet.36:526-527(2005) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] tissue: Fetal skeletal muscle.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13810708	E8282p	96T	$900		Add to Cart

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