ELISA Kit for Pig Retinoic acid receptor RXR-gamma

Mfr.No.

E13526p

Alternative Names

Nuclear receptor subfamily 2 group B member 3 ; Retinoid X receptor gamma ; NR2B3

Categories

Elisa

Function

Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid.

Specificity

Natural and recombinant Pig Retinoic acid receptor RXR-gamma

Subcellular Location

Nucleus

Entryname

RXRG_PIG

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RXRG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	95-111%	96-113%	98-110%	99-107%
EDTA plasma(n=5)	97-107%	95-107%	96-106%	98-106%
heparin plasma(n=5)	93-110%	94-109%	99-107%	93-109%

Platform

PF00104

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.9%

Protein Name

Retinoic acid receptor RXR-gamma

Recovery

Recovery was determined by spiking various levels of RXRG into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	104	95-106
Plasma	102	93-106

Sequence

50:

MYGNYSHFMK ; FPTGFGGSPG ; HSGSTSMSPS ; AALSTGKPMD ; SHPSYTDTPV ;

100:

SAPRTLSAVG ; TPLNALGSPY ; RVITSTMGPP ; SGTLAAPPGI ; NLVAPPSSQL ;

150:

NVVNSVSISE ; DIKPLPGLPG ; IGNMNYPSTS ; PGSLVKHICA ; ICGDRSSGKH ;

200:

YGVYSCEGCK ; GFFKRTIRKD ; LIYTCRDNKD ; CLIDKRQRNR ; CQYCRYQKCL ;

250:

VMGMKREAVQ ; EERQRSRERA ; ESEAECASSG ; HEDMPVERIL ; EAELAVEPKT ;

300:

ESYGDMNMEN ; STNDPVTNIC ; HAADKQLFTL ; VEWAKRIPHF ; SDLTLEDQVI ;

350:

LLRAGWNELL ; IASFSHRSVS ; VQDGILLATG ; LHVHRSSAHS ; AGVGSIFDRV ;

400:

LTELVSKMKD ; MQMDKSELGC ; LRAIVLFNPD ; AKGLSNPSEV ; ETLREKVYAT ;

450:

LEAYTKQKYP ; EQPGRFAKLL ; LRLPALRSIG ; LKCLEHLFFF ; KLIGDTPIDT ;

463:

FLMEMLETPL ; QIT

Sequence Length

463

String

Q0GFF6

Sub Unit

Homodimer (By similarity). Heterodimer with a RAR molecule (By similarity). Binds DNA preferentially as a RAR/RXR heterodimer.

Uniprot	Q0GFF6
Gene Name	RXRG
Immunogen Species	Sus Scrofa (Pig)
Kegg	ssc:445465
Unigene	Ssc.19608

Reference	1. "Cloning and expression of RXR gamma in porcine." Yu H., Song Y., Ding J., Lee L. Submitted (2006-07) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] 2. "High-resolution comparative mapping of pig Chromosome 4, emphasizing the FAT1 region." Moller M., Berg F., Riquet J., Pomp D., Archibald A., Anderson S., Feve K., Zhang Y., Rothschild M., Milan D., Andersson L., Tuggle C.K. Mamm. Genome15:717-731(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 33-463

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SKU	Supplier No.	Size	Your price	Quantity
BHE13809995	E13526p	96T	$900		Add to Cart

ELISA Kit for Pig Retinoic acid receptor RXR-gamma

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