ELISA Kit for Pig Interleukin-8

Mfr.No.

E0080p

Alternative Names

Alveolar macrophage chemotactic factor I ; AMCF-I ; C-X-C motif chemokine 8 ; Chemokine (C-X-C motif) ligand 8 ; IL8

Categories

Elisa

Function

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.

Specificity

Natural and recombinant Pig Interleukin-8

Subcellular Location

Secreted

Detection Range

15.6-1000pg/mL

Entryname

IL8_PIG

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	100-105%	97-104%	95-101%	103-106%
EDTA plasma(n=5)	98-104%	94-103%	90-97%	105-109%
heparin plasma(n=5)	94-99%	103-108%	96-104%	98-105%

Method Type

Sandwich

Platform

PF00048

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

Protein Name

Interleukin-8

Recovery

Recovery was determined by spiking various levels of IL8 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	100	97-106
Plasma	101	95-104

Sensitivity

4.3pg/mL

Sequence

50:

MTSKLAVAFL ; AVFLLSAALC ; EAAVLARVSA ; ELRCQCINTH ; STPFHPKFIK ;

100:

ELRVIESGPH ; CENSEIIVKL ; VNGKEVCLDP ; KEKWVQKVVQ ; IFLKRTEKQQ ;

103:

QQQ

Sequence Length

103

String

P26894

Sub Unit

Homodimer.

Uniprot	P26894
Gene Name	CXCL8
Immunogen Species	Sus Scrofa (Pig)
Kegg	ssc:100620730
Unigene	Ssc.658

Reference	1. "Regulation of interleukin-8 expression in porcine alveolar macrophages by bacterial lipopolysaccharide." Lin G., Pearson A.E., Scamurra R.W., Zhou Y., Baarsch M.J., Weiss D.J., Murtaugh M.P. J. Biol. Chem.269:77-85(1994) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] 2. Sanjanwala M. Submitted (1991-07) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] 3. "Molecular cloning of porcine alveolar macrophage-derived neutrophil chemotactic factors I and II; identification of porcine IL-8 and another intercrine-alpha protein." Goodman R.B., Foster D.C., Mathewes S.L., Osborn S.G., Kuijper J.L., Forstrom J.W., Martin T.R. Biochemistry31:10483-10490(1992) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PROTEIN SEQUENCE OF 26-45 tissue: Lung. 4. Goodman R.B. Submitted (1996-03) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SEQUENCE REVISION TO 23 5. "Identification of two neutrophil chemotactic peptides produced by porcine alveolar macrophages." Goodman R.B., Forstrom J.W., Osborn S.G., Chi E.Y., Martin T.R. J. Biol. Chem.266:8455-8463(1991) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 26-45 strain: Yorkshire.

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BHE13805297	E0080p	96T	$900		Add to Cart

ELISA Kit for Pig Interleukin-8

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