ELISA Kit for Pig Heat-stable enterotoxin receptor

Mfr.No.

E14905p

Alternative Names

Guanylyl cyclase C ; GC-C ; Intestinal guanylate cyclase ; GUC2C

Categories

Elisa

Function

Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptide guanylin.

Specificity

Natural and recombinant Pig Heat-stable enterotoxin receptor

Subcellular Location

Membrane Single-pass type I membrane protein

Entryname

GUC2C_PIG

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

Method Type

Sandwich

Platform

PF01094

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

Protein Name

Heat-stable enterotoxin receptor

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

Sequence

50:

MKTPLLALAL ; WSLLLQLGLT ; FWPSSVSQNC ; HNGSYEISVL ; MMNNSAFPES ;

100:

LDNLKAVVNE ; GVNIVRQRLL ; EAGLTVTVNA ; TFVYSEGVIY ; KSSDCRSSTC ;

150:

EGLDLLRTIS ; SEKRMGCVLL ; GPSCTYSTFQ ; MYLDTDLNYP ; MISAGSFGLS ;

200:

CDYKETLTRL ; MSPARKLMYF ; LVDFWKVNNF ; PFKPFSWNTA ; YVFKNSTESE ;

250:

DCFWYLNALE ; AGVSYFSQKL ; SFKEMLRGNE ; EFQNILMDQN ; RKSNVIIMCG ;

300:

APETVHTLKG ; GRAVAEDTVI ; ILVDLFNDHY ; FMDNVTAPDY ; MKNVLVLTLP ;

350:

PENSVSNSSF ; SKDLSLVKND ; FTLAYMNGVL ; LFGHMLKIFL ; EKREDVTTSK ;

400:

FAHAFRNITF ; EGHMGPVTLD ; NCGDIDNTMF ; LLYTSVDTSK ; YKVLLTYDTR ;

450:

KNYTNPVDKS ; PTFIWKNHKL ; PNDIPGRGPQ ; ILMIAVFTLT ; GTIVLLLLIA ;

500:

LLVLRKYKRE ; YALRQKKWSH ; IPPENIFPLE ; SNETNHVSLK ; IDDDRRRDTI ;

550:

QRLRQCKYDK ; KRVILKDLKH ; NDGNFTEKQK ; IELNKLLQID ; YYNLTKFYGT ;

600:

VKLDSMIFGV ; IEYCERGSLR ; EVLNDTISYP ; DGTFMDWEFK ; ISVLYDIAKG ;

650:

MSYLHSSKTE ; VHGRLKSTNC ; VVDSRMVVKI ; TDFGCNSILA ; PKKDLWTAPE ;

700:

HLRRASVSQK ; GDVYSYGIIA ; QEIILRRETF ; YTLSCRDQKE ; KIFRVENSNG ;

750:

VKPFRPDLFL ; ETAEEKELEV ; YLLVKNCWEE ; DPEKRPDFKK ; IENTLAKIFG ;

800:

LFHDQKNESY ; MDTLIRRLQL ; YSRNLEHLVE ; ERTQLYKAER ; DRADRLNFML ;

850:

LPRLVVKSLK ; EKGIVEPELY ; EEVTIYFSDI ; VGFTTICKYS ; TPMEVVDMLN ;

900:

DIYKSFDHIL ; DHHDVYKVET ; IGDAYMVASG ; LPKRNGNRHA ; IDIAKMALDI ;

950:

LSFMGTFELE ; HLPGLPIWIR ; IGIHSGPCAA ; GVVGIKMPRY ; CLFGDTVNTA ;

1000:

SRMESTGLPL ; RIHVSGSTIA ; ILKRTECQFL ; YEVRGETYLK ; GRGTETTYWL ;

1050:

TGVKDQEYNL ; PTPPTAENQQ ; RLQAEFVDMI ; ASSLQKRQAS ; GIKNRKPTRV ;

1073:

ASYKKGTLEY ; LQLNTTDNES ; THF

Sequence Length

1073

String

P55204

Sub Unit

Homotrimer. Interacts via its C-terminal region with PDZK2.

Uniprot	P55204
Gene Name	GUCY2C
Immunogen Species	Sus Scrofa (Pig)
Kegg	ssc:397193
Unigene	Ssc.310

Reference	1. "Pig intestinal membrane-bound receptor (guanylyl cyclase) for heat-stable enterotoxin: cDNA cloning, functional expression, and characterization." Wada A., Hirayama T., Kitao S., Fujisawa J., Hidaka Y., Shimonishi Y. Microbiol. Immunol.38:535-541(1994) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Small intestine. 2. Wada A. Submitted (1996-06) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SEQUENCE REVISION TO 238 AND 509

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ELISA Kit for Pig Heat-stable enterotoxin receptor

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