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ELISA Kit for Pig Glyceraldehyde-3-phosphate dehydrogenase

SKU : BHE13804592

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E1932p
Alternative Names	Peptidyl-cysteine S-nitrosylase GAPDH ; 2.6.99.- ; GAPD
Categories	Elisa
Function	Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC.
Specificity	Natural and recombinant Pig Glyceraldehyde-3-phosphate dehydrogenase
Subcellular Location	Cytoplasm cytosol Nucleus Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal.
Entryname	G3P_PIG
Platform	PF02800
Protein Name	Glyceraldehyde-3-phosphate dehydrogenase
Sequence	50: MVKVGVNGFG ; RIGRLVTRAA ; FNSGKVDIVA ; INDPFIDLHY ; MVYMFQYDST ; 100: HGKFHGTVKA ; ENGKLVINGK ; AITIFQERDP ; ANIKWGDAGA ; TYVVESTGVF ; 150: TTMEKAGAHL ; KGGAKRVIIS ; APSADAPMFV ; MGVNHEKYDN ; SLKIVSNASC ; 200: TTNCLAPLAK ; VIHDHFGIVE ; GLMTTVHAIT ; ATQKTVDGPS ; GKLWRDGRGA ; 250: AQNIIPASTG ; AAKAVGKVIP ; ELNGKLTGMA ; FRVPTPNVSV ; VDLTCRLEKP ; 300: AKYDDIKKVV ; KQASEGPLKG ; ILGYTEDQVV ; SCDFNSDTHS ; STFDAGAGIA ; 333: LNDHFVKLIS ; WYDNEFGYSN ; RVVDLMVHMA ; SKE
Sequence Length	333
String	P00355
Sub Unit	Homotetramer. Interacts with EIF1AD, USP25, PRKCI and WARS. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation.

Uniprot	P00355
Gene Name	GAPDH
Immunogen Species	Sus Scrofa (Pig)
Kegg	ssc:396823
Unigene	Ssc.16135

Reference	1. "Sus scrofa glyceraldehyde-3-phosphate dehydrogenase, genomic and cDNA sequences." Behdjani R., Silversides D.W. Submitted (1997-08) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] 2. "Evaluation and characterization of a porcine small intestine cDNA library." Winteroe A.K., Fredholm M. Submitted (1997-01) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-126 tissue: Small intestine. 3. "Glyceraldehyde 3-phosphate dehydrogenase from pig muscle." Harris J.I., Perham R.N. Nature219:1025-1028(1968) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-333 4. Harris J.I., Davidson B.E., Sajgo M., Noller H.F., Perham R.N. [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SEQUENCE REVISION TO 46 5. "Structural evidence for a liver-specific glyceraldehyde-3-phosphate dehydrogenase." Kulbe K.D., Jackson K.W., Tang J. Biochem. Biophys. Res. Commun.67:35-42(1975) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-23 tissue: Liver. 6. Foss D.L., Murtaugh M.P. Submitted (1996-02) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 15-301 7. "Detection of transcripts for retinoic acid receptors, retinol-binding protein, and transforming growth factors during rapid trophoblastic elongation in the porcine conceptus." Yelich J.V., Pomp D., Geisert R.D. Biol. Reprod.57:286-294(1997) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 174-323 8. "Isolation and characterization of cDNA clones coding for porcine muscle proteins." Davoli R., Zambonelli P., Fontanesi L., Bigi D., Costosi E., Russo V. Submitted (1995-12) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 299-333 tissue: Skeletal muscle.

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BHE13804592	E1932p	96T	$900		Add to Cart

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