ELISA Kit for Pig C-C motif chemokine 2

Mfr.No.

E0087p

Alternative Names

Monocyte chemoattractant protein 1 ; Monocyte chemotactic protein 1 ; MCP-1 ; Small-inducible cytokine A2 ; SCYA2

Categories

Elisa

Function

Chemotactic factor that attracts monocytes, but not neutrophils.

Specificity

Natural and recombinant Pig C-C motif chemokine 2

Subcellular Location

Secreted

Detection Range

31.2-2000pg/mL

Entryname

CCL2_PIG

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CCL2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-98%	96-103%	97-105%	102-108%
EDTA plasma(n=5)	96-101%	98-105%	99-106%	91-96%
heparin plasma(n=5)	90-103%	91-104%	94-105%	95-102%

Method Type

Sandwich

Platform

PF00048

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

Protein Name

C-C motif chemokine 2

Recovery

Recovery was determined by spiking various levels of CCL2 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	91-99
Plasma	96	94-103

Sensitivity

8.5pg/mL

Sequence

50:

MKVSAALLCL ; LLTAATFCTQ ; VLAQPDAINS ; PVTCCYTLTS ; KKISMQRLMS ;

99:

YRRVTSSKCP ; KEAVIFKTIA ; GKEICAEPKQ ; KWVQDSISHL ; DKKNQTPKP

Sequence Length

String

P42831

Sub Unit

Monomer or homodimer; in equilibrium.

Uniprot	P42831
Gene Name	CCL2
Immunogen Species	Sus Scrofa (Pig)
Kegg	ssc:397422
Unigene	Ssc.657

Reference	1. "Porcine luteal cells express monocyte chemoattractant protein-1 (MCP-1): analysis by polymerase chain reaction and cDNA cloning." Hosang K., Knoke I., Klaudiny J., Wempe F., Wuttke W., Scheit K.H. Biochem. Biophys. Res. Commun.199:962-968(1994) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] 2. Zach O.R.F. Submitted (1994-07) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Brain.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13801865	E0087p	96T	$900		Add to Cart

ELISA Kit for Pig C-C motif chemokine 2

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