ELISA Kit for Pig 5'-AMP-activated protein kinase catalytic subunit alpha-1

Mfr.No.

E9709p

Alternative Names

AMPK 63 kDa subunit ; Acetyl-CoA carboxylase kinase ; ACACA kinase ; 2.7.11.27 ; Hydroxymethylglutaryl-CoA reductase kinase ; HMGCR kinase ; 2.7.11.31 ; Tau-protein kinase PRKAA1 ; 2.7.11.26 ; AMPK1

Categories

Elisa

Function

Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1.

Specificity

Natural and recombinant Pig 5'-AMP-activated protein kinase catalytic subunit alpha-1

Subcellular Location

Cytoplasm Nucleus In response to stress, recruited by p53/TP53 to specific promoters.

Entryname

AAPK1_PIG

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AAPK1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	87-104%	91-100%	90-102%	89-99%
EDTA plasma(n=5)	89-105%	93-103%	94-100%	92-104%
heparin plasma(n=5)	91-102%	90-106%	91-102%	91-103%

Method Type

Sandwich

Platform

PF00069

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.9%

Protein Name

5'-AMP-activated protein kinase catalytic subunit alpha-1

Recovery

Recovery was determined by spiking various levels of AAPK1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	95	88-101
Plasma	97	90-103

Sequence

50:

DGRVKIGHYI ; LGDTLGVGTF ; GKRREIQNLK ; LFRHPHIIKL ; YQVISTPSDI ;

100:

FMVMEYVSGG ; ELFDYICKNG ; RLDEKESRRL ; FQQILSGVDY ; CHRHMVVHRD ;

150:

LKPENVLLDA ; HMNAKIADFG ; LSNMMSDGEF ; LRTSCGSPNY ; AAPEVISGRL ;

200:

YAGPEVDIWS ; SGVILYALLC ; GTLPFDDDHV ; PTLFKKICDG ; IFYTPQYLNP ;

250:

SVISLLKHML ; QVDPMKRATI ; KDIREHEWFK ; QDLPKYLFPE ; DPSYSXTMID ;

300:

DEALKQDPLA ; VAYHLIIDNR ; DFYLATSPPD ; SFLDDHHLTR ; VPFLVAETPR ;

350:

DELNPQKXKH ; QGVRKAKXHL ; GIRQLDYEXK ; VVNPYYLRVR ; RKKMSLQLYQ ;

385:

VDSRTYLLDF ; RSIDDXIDAE ; AQGKSSEASL ; TXSVT

Sequence Length

385

Sub Unit

AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.

Uniprot	Q09136
Gene Name	PRKAA1
Immunogen Species	Sus Scrofa (Pig)
Unigene	Ssc.26892

Reference	1. "Mammalian AMP-activated protein kinase shares structural and functional homology with the catalytic domain of yeast Snf1 protein kinase." Mitchelhill K.I., Stapleton D., Gao G., House C., Michell B., Katsis F., Witters L.A., Kemp B.E. J. Biol. Chem.269:2361-2364(1994) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 1-22; 73-87; 100-157; 222-240 AND 344-361;FUNCTION tissue: Liver. 2. "Mammalian AMP-activated protein kinase subfamily." Stapleton D., Mitchelhill K.I., Gao G., Widmer J., Michell B.J., Teh T., House C.M., Fernandez C.S., Cox T., Witters L.A., Kemp B.E. J. Biol. Chem.271:611-614(1996) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 1-22; 73-88; 100-132; 144-156; 209-216; 221-255; 256-270; 271-290; 291-300; 301-323; 324-342; 343-367 AND 368-385 tissue: Liver. 3. "Effect of dietary hemicellulose on growth and energy metabolism of growing pigs." Weber T.E. Submitted (2008-04) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 23-243

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SKU	Supplier No.	Size	Your price	Quantity
BHE13800440	E9709p	96T	$900		Add to Cart

ELISA Kit for Pig 5'-AMP-activated protein kinase catalytic subunit alpha-1

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