ELISA Kit for Mouse Vascular endothelial growth factor A

Mfr.No.

E0143m

Alternative Names

Vascular permeability factor ; VPF ; Vegf

Categories

Elisa

Function

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis.

Specificity

Natural and recombinant Mouse Vascular endothelial growth factor A

Subcellular Location

Isoform VEGF-3 Cell membrane Peripheral membrane protein Remains cell-surface associated unless released by heparin.

Detection Range

15.6-1000pg/mL

Entryname

VEGFA_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGF-A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	84-93%	79-94%	88-96%	78-91%
EDTA plasma(n=5)	78-89%	82-97%	90-102%	89-103%
heparin plasma(n=5)	83-94%	89-101%	87-99%	91-103%

Method Type

Sandwich

Platform

PF00341

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 4.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 10.2%

Protein Name

Vascular endothelial growth factor A

Recovery

Recovery was determined by spiking various levels of VEGF-A into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	86	80-95
Plasma	95	90-102

Sensitivity

4.16pg/mL

Sequence

50:

MNFLLSWVHW ; TLALLLYLHH ; AKWSQAAPTT ; EGEQKSHEVI ; KFMDVYQRSY ;

100:

CRPIETLVDI ; FQEYPDEIEY ; IFKPSCVPLM ; RCAGCCNDEA ; LECVPTSESN ;

150:

ITMQIMRIKP ; HQSQHIGEMS ; FLQHSRCECR ; PKKDRTKPEK ; KSVRGKGKGQ ;

200:

KRKRKKSRFK ; SWSVHCEPCS ; ERRKHLFVQD ; PQTCKCSCKN ; TDSRCKARQL ;

214:

ELNERTCRCD ; KPRR

Sequence Length

214

String

Q00731

Sub Unit

Homodimer; disulfide-linked. Also found as heterodimer with PGF.

Uniprot	Q00731
Gene Name	Vegfa
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:22339
Unigene	Mm.282184

Reference	1. "Expression of vascular endothelial growth factor during embryonic angiogenesis and endothelial cell differentiation." Breier G., Albrecht U., Sterrer S., Risau W. Development114:521-532(1992) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS VEGF-1; VEGF-2 AND VEGF-3) 2. "Vascular endothelial growth factor. Regulation by cell differentiation and activated second messenger pathways." Claffey K.P., Wilkison W.O., Spiegelman B.M. J. Biol. Chem.267:16317-16322(1992) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM VEGF-1) 3. "A novel alternatively spliced form of murine vascular endothelial growth factor, VEGF 115." Sugihara T., Wadhwa R., Kaul S.C., Mitsui Y. J. Biol. Chem.273:3033-3038(1998) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM VEGF-4) strain: ICR. 4. "Identification of a unique Mus musculus VEGF isoform of 102 amino acids." Jankowsky J.A., Adamski F.M., Robertson F.M. Submitted (2003-03) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM VEGF-5) strain: FVB/N. 5. "Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S., Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.more... PLoS Biol.7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] strain: C57BL/6J. 6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PARTIAL NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM L-VEGF-1) strain: C57BL/6. tissue: Brain. 7. "Alternatively spliced forms of mouse VEGF." Minchenko O.H., Minchenko D.O., Opentanova I.L. Submitted (2004-09) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-123 (ISOFORMS VEGF-1/VEGF-2/VEGF-3) strain: C57BL/6. tissue: Retina. 8. "The mouse gene for vascular endothelial growth factor. Genomic structure, definition of the transcriptional unit, and characterization of transcriptional and post-transcriptional regulatory sequences." Shima D.T., Kuroki M., Deutsch U., Ng Y., Adamis A.P., D'Amore P.A. J. Biol. Chem.271:3877-3883(1996) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-3 (ISOFORMS VEGF-1/VEGF-2/VEGF-3/VEGF-4/VEGF-5) 9. "Regulation of VEGF and VEGF receptor expression in the rodent mammary gland during pregnancy, lactation, and involution." Pepper M.S., Baetens D., Mandriota S.J., Di Sanza C., Oikemus S., Lane T.F., Soriano J.V., Montesano R., Iruela-Arispe M.L. Dev. Dyn.218:507-524(2000) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DEVELOPMENTAL STAGE 10. "The regulators of VEGF expression in mouse ovaries." Shin S.-Y., Lee H.-J., Ko D.-S., Lee H.-C., Park W.I. Yonsei Med. J.46:679-686(2005) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INDUCTION

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ELISA Kit for Mouse Vascular endothelial growth factor A

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