ELISA Kit for Mouse Uveal autoantigen with coiled-coil domains and ankyrin repeats

Mfr.No.

E3054m

Alternative Names

Nuclear membrane-binding protein ; Nucling ; Kiaa1561

Categories

Elisa

Function

Modulates isoactin dynamics to regulate the morphological alterations required for cell growth and motility. Interaction with ARF6 may modulate cell shape and motility after injury.

Specificity

Natural and recombinant Mouse Uveal autoantigen with coiled-coil domains and ankyrin repeats

Subcellular Location

Nucleus Cytoplasm Cytoplasm cytoskeleton Expressed diffusely in cytoplasm.

Detection Range

0.312-20ng/mL

Entryname

UACA_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of UACA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	91-107%	93-103%	94-101%	92-105%
EDTA plasma(n=5)	90-110%	92-105%	95-103%	94-104%
heparin plasma(n=5)	93-104%	94-106%	92-108%	91-109%

Method Type

Competitive

Platform

PF00023

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.9%

Protein Name

Uveal autoantigen with coiled-coil domains and ankyrin repeats

Recovery

Recovery was determined by spiking various levels of UACA into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	98	91-104
Plasma	99	92-105

Sensitivity

0.18ng/mL

Sequence

50:

MKSLKSRLWK ; QDAPGPTSPS ; SPTAVASTQS ; AEWNKYDDRL ; MKAAERGDVE ;

100:

KVSSILAKKG ; VHPGKLDVEG ; RSAFHVVASK ; GNLECLNAIL ; THGIDVATRD ;

150:

SAGRNALHLA ; AKYGHALCLQ ; KLLQYNCPTE ; HVDLQGRTAL ; HDAVMADCPS ;

200:

SIQLLCDHGA ; SVNAKDIDGR ; TPLVLATQMC ; RPTICQLLID ; RGADVNSRDK ;

250:

QNRTALMLGC ; EYGCRDAVEV ; LVKNGADLTL ; LDALGHDSSY ; YARIGDNLDI ;

300:

LNLLKTASEN ; TNKGRELWRK ; GPPLQQRNLS ; HTQDEGSVKS ; TQREQREPHS ;

350:

FQDLEIENED ; LREKLRKIQQ ; EQRILLDKVN ; GLQLQLNEEV ; MVADDLESER ;

400:

EKPKSLLAAK ; EKQHEESLRT ; IEALKNRFKY ; FESDHPGPGS ; YPSNRKEDML ;

450:

HKQGQMYTTE ; PQCASPGIPP ; HMHSRSMLRP ; LELSLPSQTS ; YSENEILKKE ;

500:

LETLRTYYDS ; AKQDRLKFQN ; ELAHKVAECK ; ALALECERVK ; EDSDEQIKQL ;

550:

EDALKDVQKR ; MYESEGKVKQ ; MQTHFLALKE ; HLTNEAATGS ; HRIIEELREQ ;

600:

LKDLKGKYEG ; ASAEVGKLRS ; QIKQSEMLVG ; EFKRDEGRLV ; EENKRLQKEC ;

650:

GTCEVELERR ; GRRVVELEGQ ; LKELGAKLAL ; SVPTEKFESM ; KSSLSNDINE ;

700:

KVKRLAEVGR ; DYESAQGEIR ; QLKRDLESVR ; AQHIRPEEHE ; QLRSRLEQKS ;

750:

GELGKKVSEL ; TLKNQTLQKD ; VEKLHADNKL ; LNQQVHSLTV ; EMKTRYVPLR ;

800:

VSEEMKRSHD ; VNVEDLNKKL ; SEATQRYAEK ; KQEAERLLAE ; NDKLTKNVSR ;

850:

LEAVFVAPEK ; HEKELMGLKS ; NIAELKKQLS ; ELNKKCGEGQ ; EKIRALMSEN ;

900:

SSLKKTLSSQ ; YVPAKTHEEV ; KASLNSTVEK ; TNRALLEAKK ; RFDDTSQEVS ;

950:

KLRDENEVLR ; RNLENVQNQM ; KADYVSLEEH ; SRRMSTVSQS ; LKEAQEANAA ;

1000:

ILADHRQGQE ; EIVSLHAEIK ; AQKKELDTIQ ; ECIKLKYAPL ; ARLEECERKF ;

1050:

KATEKGLKEQ ; LSEQTHKCRQ ; RDEEVKKGKQ ; ENERLRADLA ; ALQKELQDRN ;

1100:

ALAEEAREAE ; RALSGKADEL ; SKQLKDLSQK ; YSDVKSEREK ; LVEEKAKQAS ;

1150:

EILAAQNLLQ ; KQPVPLEQVE ; ALKKSLNGTI ; EQLKEELRSK ; QRCLEREQQT ;

1200:

VSQLQQLLEN ; QKNSSVTLAE ; HLKLKEALEK ; EVGIMKASLR ; EKEEESQKKT ;

1250:

KEVSKLQTEV ; QTTKQALKNL ; ETREVVDMSK ; YKATKNDLET ; QISNLNDKLA ;

1300:

SLNRKYDQAC ; EEKVSAKDEK ; ELLHLSIEQE ; IRDQKERCDK ; SLTTIMELQQ ;

1350:

RIQESAKQIE ; AKDNKITELL ; NDVERLKQAL ; NGLSQLTYSS ; GSPTKRQSQL ;

1400:

VDTLQQRVRD ; LQQQLADADR ; QHQEVIAIYR ; THLLSAAQGH ; MDEDVQAALL ;

1411:

QIIQMRQGLV ; C

Sequence Length

1411

String

Q8CGB3

Sub Unit

Component of the apoptosome complex, composed of APAF1, pro-caspase-9 and UACA. In the complex, it probably interacts directly with APAF1. Interacts with LGALS3, ARF6 and ACTB.

Uniprot	Q8CGB3
Gene Name	Uaca
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:72565
Unigene	Mm.68819

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ELISA Kit for Mouse Uveal autoantigen with coiled-coil domains and ankyrin repeats

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