ELISA Kit for Mouse Ubiquitin-like protein ISG15

Mfr.No.

E14926m

Alternative Names

Interferon-induced 15 kDa protein ; Interferon-induced 17 kDa protein ; IP17 ; Ubiquitin cross-reactive protein ; G1p2 ; Ucrp

Categories

Elisa

Function

In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy.

Specificity

Natural and recombinant Mouse Ubiquitin-like protein ISG15

Subcellular Location

Cytoplasm Secreted UCRP conjugates seem to be noncovalently associated with the intermediate filaments and distributed in a punctate pattern. Also secreted.

Detection Range

78-5000pg/mL

Entryname

ISG15_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ISG15 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	99-108%	98-111%	100-106%	99-109%
EDTA plasma(n=5)	95-111%	97-108%	98-108%	100-107%
heparin plasma(n=5)	98-106%	94-112%	96-109%	98-108%

Method Type

Sandwich

Platform

PF00240

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.3%

Protein Name

Ubiquitin-like protein ISG15

Recovery

Recovery was determined by spiking various levels of ISG15 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	104	96-107
Plasma	103	94-106

Sensitivity

39.3pg/mL

Sequence

50:

MAWDLKVKML ; GGNDFLVSVT ; NSMTVSELKK ; QIAQKIGVPA ; FQQRLAHQTA ;

100:

VLQDGLTLSS ; LGLGPSSTVM ; LVVQNCSEPL ; SILVRNERGH ; SNIYEVFLTQ ;

150:

TVDTLKKKVS ; QREQVHEDQF ; WLSFEGRPME ; DKELLGEYGL ; KPQCTVIKHL ;

161:

RLRGGGGDQC ; A

Sequence Length

161

String

Q64339

Sub Unit

Interacts with, and is conjugated to its targets by the UBE1L (E1 enzyme) and UBE2E2 (E2 enzyme).

Uniprot	Q64339
Gene Name	Isg15
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:100038882
Unigene	Mm.451527

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SKU	Supplier No.	Size	Your price	Quantity
BHE13805128	E14926m	96T	$776		Add to Cart

ELISA Kit for Mouse Ubiquitin-like protein ISG15

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