ELISA Kit for Mouse Tyrosine-protein kinase Tec

Mfr.No.

E8704m

Categories

Elisa

Function

Non-receptor tyrosine kinase that contributes to signaling from many receptors and participates as a signal transducer in multiple downstream pathways, including regulation of the actin cytoskekleton. Plays a redundant role to ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. Required for TCR-dependent IL2 gene induction. Phosphorylates DOK1, one CD28-specific substrate, and contributes to CD28-signaling. Mediates signals that negatively regulate IL2RA expression induced by TCR cross-linking. Plays a redundant role to BTK in BCR-signaling for B-cell development and activation, especially by phosphorylating STAP1, a BCR-signaling protein. Required in mast cells for efficient cytokine production. Involved in both growth and differentiation mechanisms of myeloid cells through activation by the granulocyte colony-stimulating factor CSF3, a critical cytokine to promoting the growth, differentiation, and functional activation of myeloid cells. Participates in platelet signaling downstream of integrin activation. Cooperates with JAK2 through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. GRB10, a negative modifier of the FOS activation pathway, is another substrate of TEC. TEC is involved in G protein-coupled receptor- and integrin-mediated signalings in blood platelets. Plays a role in hepatocyte proliferation and liver regeneration and is involved in HGF-induced ERK signaling pathway. TEC regulates also FGF2 unconventional secretion (endoplasmic reticulum (ER)/Golgi-independent mechanism) under various physiological conditions through phosphorylation of FGF2 'Tyr-82'. May also be involved in the regulation of osteoclast differentiation.

Specificity

Natural and recombinant Mouse Tyrosine-protein kinase Tec

Subcellular Location

Cytoplasm Cell membrane Peripheral membrane protein Cytoplasm cytoskeleton Following B-cell or T-cell receptors activation by antigen, translocates to the plasma membrane through its PH domain. Thrombin and integrin engagement induces translocation of TEC to the cytoskeleton during platelet activation. In cardiac myocytes, assumes a diffuse intracellular localization under basal conditions but is recruited to striated structures upon various stimuli, including ATP.

Detection Range

0.156-10ng/mL

Entryname

TEC_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TEC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-99%	90-101%	91-98%	92-97%
EDTA plasma(n=5)	90-102%	93-100%	89-104%	93-98%
heparin plasma(n=5)	93-97%	91-103%	92-100%	91-102%

Method Type

Sandwich

Platform

PF00779

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.9%

Protein Name

Tyrosine-protein kinase Tec

Recovery

Recovery was determined by spiking various levels of TEC into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	95	91-99
Plasma	96	93-101

Sensitivity

0.078ng/mL

Sequence

50:

MNFNTILEEI ; LIKRSQQKKK ; TSLLNYKERL ; CVLPKSVLSY ; YEGRAEKKYR ;

100:

KGVIDISKIK ; CVEIVKNDDG ; VIPCQNKFPF ; QVVHDANTLY ; IFAPSPQSRD ;

150:

RWVKKLKEEI ; KNNNNIMIKY ; HPKFWADGSY ; QCCRQTEKLA ; PGCEKYNLFE ;

200:

SSIRKTLPPA ; PEIKKRRPPP ; PIPPEEENTE ; EIVVAMYDFQ ; ATEAHDLRLE ;

250:

RGQEYIILEK ; NDLHWWRARD ; KYGSEGYIPS ; NYVTGKKSNN ; LDQYEWYCRN ;

300:

TNRSKAEQLL ; RTEDKEGGFM ; VRDSSQPGLY ; TVSLYTKFGG ; EGSSGFRHYH ;

350:

IKETATSPKK ; YYLAEKHAFG ; SIPEIIEYHK ; HNAAGLVTRL ; RYPVSTKGKN ;

400:

APTTAGFSYD ; KWEINPSELT ; FMRELGSGLF ; GVVRLGKWRA ; QYKVAIKAIR ;

450:

EGAMCEEDFI ; EEAKVMMKLT ; HPKLVQLYGV ; CTQQKPIYIV ; TEFMERGCLL ;

500:

NFLRQRQGHF ; SRDMLLSMCQ ; DVCEGMEYLE ; RNSFIHRDLA ; ARNCLVNEAG ;

550:

VVKVSDFGMA ; RYVLDDQYTS ; SSGAKFPVKW ; CPPEVFNYSR ; FSSKSDVWSF ;

600:

GVLMWEIFTE ; GRMPFEKNTN ; YEVVTMVTRG ; HRLHRPKLAT ; KYLYEVMLRC ;

630:

WQERPEGRPS ; LEDLLRTIDE ; LVECEETFGR ;

Sequence Length

630

String

P24604

Sub Unit

Interacts with INPP5D/SHIP1 and INPPL1/SHIP2. Interacts with CD28, FASLG, FGF2, GRB10 and KIT (By similarity). Interacts with VAV1 and JAK2.

Uniprot	P24604
Gene Name	Tec
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:21682
Unigene	Mm.319581

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ELISA Kit for Mouse Tyrosine-protein kinase Tec

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