ELISA Kit for Mouse Tumor necrosis factor

Mfr.No.

E0133m

Alternative Names

Cachectin ; TNF-alpha ; Tumor necrosis factor ligand superfamily member 2 ; TNF-a ; Tnfa ; Tnfsf2

Categories

Elisa

Function

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Specificity

Natural and recombinant Mouse Tumor necrosis factor

Subcellular Location

Tumor necrosis factor, soluble form Secreted

Detection Range

7.8-500pg/mL

Entryname

TNFA_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-101%	96-105%	98-104%	98-107%
EDTA plasma(n=5)	95-103%	97-107%	100-110%	88-103%
heparin plasma(n=5)	98-103%	96-102%	93-104%	95-115%

Method Type

Sandwich

Platform

PF00229

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.4%

Protein Name

Tumor necrosis factor

Recovery

Recovery was determined by spiking various levels of TNF into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	102	95-114
Plasma	96	93-99

Sensitivity

4.15pg/mL

Sequence

50:

MSTESMIRDV ; ELAEEALPQK ; MGGFQNSRRC ; LCLSLFSFLL ; VAGATTLFCL ;

100:

LNFGVIGPQR ; DEKFPNGLPL ; ISSMAQTLTL ; RSSSQNSSDK ; PVAHVVANHQ ;

150:

VEEQLEWLSQ ; RANALLANGM ; DLKDNQLVVP ; ADGLYLVYSQ ; VLFKGQGCPD ;

200:

YVLLTHTVSR ; FAISYQEKVN ; LLSAVKSPCP ; KDTPEGAELK ; PWYEPIYLGG ;

235:

VFQLEKGDQL ; SAEVNLPKYL ; DFAESGQVYF ; GVIAL

Sequence Length

235

String

P06804

Sub Unit

Homotrimer.

Uniprot	P06804
Gene Name	Tnf
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:21926
Unigene	Mm.1293

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SKU	Supplier No.	Size	Your price	Quantity
BHE13811463	E0133m	96T	$776		Add to Cart

ELISA Kit for Mouse Tumor necrosis factor

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